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<p>FOXL2 and CYP19B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development. The potential roles of the <i>foxl2</i> and <i>cyp19b</i> genes in sex determination and gonadal development in <i>Cyprinus carpio</i> var. <i>koi</i> were explored using a non-invasive RNA interference (RNAi) method, histopathological observation and qPCR. Results demonstrate that <i>foxl2</i> exhibited a sexually dimorphic expression pattern in gonads, with a notable expression in ovaries; <i>cyp19b</i> was expressed in all peripheral tissues, with a particularly prominent expression in brain and gonads. The knockdown of <i>foxl2</i> by RNAi resulted in delay in the development of the female gonads. Conversely, no notable alterations were discerned in the gonads of <i>C. carpio</i> var. <i>koi</i> following the knockdown of <i>cyp19b</i>. The upregulation of <i>sox9a, amh</i>, and <i>cyp19b</i> following <i>foxl2</i> knockdown indicates that <i>foxl2</i> may play a pivotal role in gonadal development. Nevertheless, further investigation is required to ascertain the potential role of <i>cyp19b</i>. This study elucidated the role of <i>foxl2</i> and enhanced the understanding of the mechanisms of sex determination and gonadal development in <i>C. carpio</i> var. <i>koi</i>.</p>

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RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads

  • 期刊论文

  • 发表日期：

    2025年1月2日

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  • Xiao Mei,
  • Xinglei Wang,
  • Qiang Zhou,
  • Bo Liu,
  • Yishun Wang,
  • Liuchang Kong,
  • Shibo Jiang,
  • Feng Wang

摘要

FOXL2 and CYP19B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development. The potential roles of the foxl2 and cyp19b genes in sex determination and gonadal development in Cyprinus carpio var. koi were explored using a non-invasive RNA interference (RNAi) method, histopathological observation and qPCR. Results demonstrate that foxl2 exhibited a sexually dimorphic expression pattern in gonads, with a notable expression in ovaries; cyp19b was expressed in all peripheral tissues, with a particularly prominent expression in brain and gonads. The knockdown of foxl2 by RNAi resulted in delay in the development of the female gonads. Conversely, no notable alterations were discerned in the gonads of C. carpio var. koi following the knockdown of cyp19b. The upregulation of sox9a, amh, and cyp19b following foxl2 knockdown indicates that foxl2 may play a pivotal role in gonadal development. Nevertheless, further investigation is required to ascertain the potential role of cyp19b. This study elucidated the role of foxl2 and enhanced the understanding of the mechanisms of sex determination and gonadal development in C. carpio var. koi.