Scholarlink

<p>The presented work aimed to develop and evaluate a novel hybrid nanocomposite scaffold for bone regeneration, integrating bovine bone-derived nano-hydroxyapatite (nHA), chitosan, and zinc oxide (ZnO) nanostructures. Natural nHA was synthesized via calcination of bovine bone at 1000&#xa0;°C, while ZnO nanorods were prepared via a sol-gel method. A composite scaffold (75 wt% nHA, 5 wt% ZnO in chitosan matrix) was fabricated. Comprehensive characterization using XRD, FTIR, TEM, and SEM/EDS confirmed the formation of enhanced crystallinity resulting from high-temperature calcination, stoichiometric nHA (~ 30&#xa0;nm crystallites) and phase-pure ZnO nanorods (30–40&#xa0;nm diameter). In vivo efficacy was assessed in a rabbit tibial defect model over six weeks. Radiographic and histopathological analyses demonstrated that defects implanted with the composite exhibited enhanced radiopacity and defect filling compared to empty controls. Histology revealed integration of the composite material, bordered by inflammatory and giant cells, with dense collagen deposition. The composite elicited a favorable biological response, supporting its potential as an osteoconductive and antibacterial bone graft substitute that combines the bioactivity of natural HA, the structural benefits of chitosan, and the osteogenic/antibacterial properties of ZnO.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synthesis, characterization, and in vivo evaluation of a bovine bone-derived nano-hydroxyapatite/chitosan composite for bone regeneration

  • 期刊论文

  • 发表日期：

    2026年5月19日

查看全文
  • Mohammed Elywa,
  • Yara Sallam,
  • Amr M. Abdelghany,
  • Mustafa Abd El Raouf,
  • Mohamed Gomaa,
  • Omar F. Farag

摘要

The presented work aimed to develop and evaluate a novel hybrid nanocomposite scaffold for bone regeneration, integrating bovine bone-derived nano-hydroxyapatite (nHA), chitosan, and zinc oxide (ZnO) nanostructures. Natural nHA was synthesized via calcination of bovine bone at 1000 °C, while ZnO nanorods were prepared via a sol-gel method. A composite scaffold (75 wt% nHA, 5 wt% ZnO in chitosan matrix) was fabricated. Comprehensive characterization using XRD, FTIR, TEM, and SEM/EDS confirmed the formation of enhanced crystallinity resulting from high-temperature calcination, stoichiometric nHA (~ 30 nm crystallites) and phase-pure ZnO nanorods (30–40 nm diameter). In vivo efficacy was assessed in a rabbit tibial defect model over six weeks. Radiographic and histopathological analyses demonstrated that defects implanted with the composite exhibited enhanced radiopacity and defect filling compared to empty controls. Histology revealed integration of the composite material, bordered by inflammatory and giant cells, with dense collagen deposition. The composite elicited a favorable biological response, supporting its potential as an osteoconductive and antibacterial bone graft substitute that combines the bioactivity of natural HA, the structural benefits of chitosan, and the osteogenic/antibacterial properties of ZnO.