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<p>This research introduces a new two-stage microfluidic channel designed to enhance the quality of sperm separation and washing. Two microchannels were created using polymethyl methacrylate (PMMA) through laser engraving and then bonded thermally. The proposed microfluidic system uses a combination of spiral and zigzag geometries to improve sperm sorting based on sperm motility and Dean forces in spiral channels. The experiment involved injecting semen and medium into the channels at different flow rates (2–13&#xa0;ml/min) and then analyzing the sperm to assess count, motility, and morphology. Experimental results demonstrate that the two-stage microchannel considerably enhances sperm separation efficiency, achieving higher recovery rates of motile sperm and better morphological quality than conventional methods. Given that the current sperm preparation techniques often compromise DNA integrity and viability, leading to suboptimal outcomes in procedures like intracytoplasmic sperm injection (ICSI). The SDF test revealed that the impact of the built microchannel on sperm DNA damage is substantially less than that of conventional sperm washing methods.</p>

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A novel two-stage microfluidic channel for high-quality sperm separation and washing

  • 期刊论文

  • 发表日期：

    2025年7月14日

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  • Saeed Derakhshan,
  • Ataallah Kamyabi,
  • Sareh Ashourzadeh,
  • Tooraj Reza Mirshekari

摘要

This research introduces a new two-stage microfluidic channel designed to enhance the quality of sperm separation and washing. Two microchannels were created using polymethyl methacrylate (PMMA) through laser engraving and then bonded thermally. The proposed microfluidic system uses a combination of spiral and zigzag geometries to improve sperm sorting based on sperm motility and Dean forces in spiral channels. The experiment involved injecting semen and medium into the channels at different flow rates (2–13 ml/min) and then analyzing the sperm to assess count, motility, and morphology. Experimental results demonstrate that the two-stage microchannel considerably enhances sperm separation efficiency, achieving higher recovery rates of motile sperm and better morphological quality than conventional methods. Given that the current sperm preparation techniques often compromise DNA integrity and viability, leading to suboptimal outcomes in procedures like intracytoplasmic sperm injection (ICSI). The SDF test revealed that the impact of the built microchannel on sperm DNA damage is substantially less than that of conventional sperm washing methods.