<p>The reindeer is a quintessential migratory species adept at traversing complex terrains, demonstrating exceptional efficiency and stability while walking and running on sloped surfaces. One of the key factors influencing its locomotion is the coordinated movement of its limbs. To investigate the biological mechanisms underpinning their remarkable locomotive performance, this study utilized high-speed imaging to capture kinematic data of reindeer fore and hind hooves on varying inclines (0°, + 12°, + 15°, + 17°, −12°, −15°, and −17°). Results revealed that, whether on level ground, inclines, or declines, reindeer alternate between static and diagonal gaits, adapting by increasing joint flexion to mitigate the “pitching anteriorly” on slopes. During uphill locomotion, reindeer enhance landing stability by increasing duty cycle and decreasing the range of motion (ROM) of the forelimb carpal joints, while coordinating hind hoof joint movements to maximize propulsion and minimize energy expenditure. When descending slopes, reindeer enhance braking effectiveness by increasing stride length and adjusting carpal joint angles, thereby controlling movement speed and absorbing impact forces, while simultaneously limiting ROM in the hind limb joints to conserve energy. This study elucidates the kinematic mechanisms by which reindeer achieve coordinated fore and hind hoof movements to enhance stability and reduce energy expenditure on sloped terrains. The findings offer crucial theoretical and technical insights into the reindeer’s ability to adapt to complex landscapes during long-distance migration.</p>