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<p>The dehydration-responsive element binding factor 2B (DREB2B) transcription factor plays a critical role in plant responses to drought, salinity, and low-temperature stress. By searching raw whole-genome and transcriptome sequencing data, we have for the first time predicted two allelic variants and sequenced the <i>DaDREB2B</i> gene of the Antarctic grass <i>Deschampsia antarctica</i>. The identity of the two predicted gene variants was 93.3%, and the sequenced variant showed an identity of 99.9% with one of the predicted alleles. We revealed that <i>DaDREB2B</i> may undergo alternative splicing, resulting in the formation of four transcript types. Among these, three encode full-length proteins, whereas the fourth features a premature stop codon, resulting in a truncated protein lacking functional domains. Unlike most other grasses, this transcript contains an alternative start codon at the start of the second exon and an open reading frame, encoding a potentially functional protein with an altered N-terminal sequence, a DNA-binding AP2/ERF domain, a nuclear localization signal, and a transcriptional activation domain. Transcriptomic data analysis revealed that this specific transcript is predominant in various tissues of plants grown both in the laboratory and under natural conditions. We characterized in detail the predicted DaDREB2B TF isoforms via bioinformatics methods. This study enhances our understanding of the mechanisms by which grasses may respond to abiotic stress and lays the groundwork for enhancing the stress resistance of agricultural crops through transgenesis.</p>

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Stress-induced transcription factor DaDREB2B in Deschampsia antarctica: potential implications of gene polymorphisms and alternative transcripts

  • 期刊论文

  • 发表日期：

    2025年5月15日

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  • O. M. Bublyk,
  • I. O. Andreev,
  • V. M. Mel’nyk,
  • V. A. Kunakh

摘要

The dehydration-responsive element binding factor 2B (DREB2B) transcription factor plays a critical role in plant responses to drought, salinity, and low-temperature stress. By searching raw whole-genome and transcriptome sequencing data, we have for the first time predicted two allelic variants and sequenced the DaDREB2B gene of the Antarctic grass Deschampsia antarctica. The identity of the two predicted gene variants was 93.3%, and the sequenced variant showed an identity of 99.9% with one of the predicted alleles. We revealed that DaDREB2B may undergo alternative splicing, resulting in the formation of four transcript types. Among these, three encode full-length proteins, whereas the fourth features a premature stop codon, resulting in a truncated protein lacking functional domains. Unlike most other grasses, this transcript contains an alternative start codon at the start of the second exon and an open reading frame, encoding a potentially functional protein with an altered N-terminal sequence, a DNA-binding AP2/ERF domain, a nuclear localization signal, and a transcriptional activation domain. Transcriptomic data analysis revealed that this specific transcript is predominant in various tissues of plants grown both in the laboratory and under natural conditions. We characterized in detail the predicted DaDREB2B TF isoforms via bioinformatics methods. This study enhances our understanding of the mechanisms by which grasses may respond to abiotic stress and lays the groundwork for enhancing the stress resistance of agricultural crops through transgenesis.