Scholarlink

Key message <p>Salt stress induces ABI5 → ALKBH10B transcription; the demethylase removes m<sup>6</sup>A from <i>RAP2.6</i> mRNA, accelerating its decay and attenuating salt-responsive genes, thus linking ABA signaling to reversible m<sup>6</sup>A control of <i>Arabidopsis</i> salt tolerance.</p> Abstract <p><i>N</i><sup>6</sup>-methyladenosine (m<sup>6</sup>A) is the most prevalent internal modification of RNA and plays an important role in regulating RNA metabolism that governs development and environmental adaptation of plants. Here we dissect how the <i>Arabidopsis</i> m<sup>6</sup>A demethylase ALKBH10B is integrated into abscisic-acid (ABA)-mediated salt-stress signaling. Under salt treatment, loss of ALKBH10B exhibited significantly delayed seed germination. Salt stress could significantly induce the expression of <i>ALKBH10B</i> transcription via the ABA-responsive transcription factor ABI5, which binds directly bind to the ABRE element in the <i>ALKBH10B</i> promoter and activate its transcription. Multi-omic integration of m<sup>6</sup>A methylomes and transcriptome identified the AP2/ERF transcription factor RAP2.6 as a direct target of ALKBH10B. ALKBH10B removed m<sup>6</sup>A modifications on <i>RAP2.6</i> mRNA, accelerating its degradation and modulating the expression of salt-responsive genes. In summary, this study elucidates the salt stress response pathway ABI5-ALKBH10B-RAP2.6 that couples ABA perception to reversible m<sup>6</sup>A modification, providing mechanistic insight into the intricate regulatory network of dynamic m<sup>6</sup>A modifications in plant stress adaptation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

ABI5-activated ALKBH10B demethylates RAP2.6 mRNA to modulate Arabidopsis salt tolerance

  • 期刊论文

  • 发表日期：

    2026年7月27日

查看全文
  • Shurong Xuan,
  • Mengxue Cheng,
  • Yunze Wen,
  • Qiang Wei,
  • Dan Zhang,
  • Peng He,
  • Jianing Yu

摘要

Key message

Salt stress induces ABI5 → ALKBH10B transcription; the demethylase removes m6A from RAP2.6 mRNA, accelerating its decay and attenuating salt-responsive genes, thus linking ABA signaling to reversible m6A control of Arabidopsis salt tolerance.

Abstract

N6-methyladenosine (m6A) is the most prevalent internal modification of RNA and plays an important role in regulating RNA metabolism that governs development and environmental adaptation of plants. Here we dissect how the Arabidopsis m6A demethylase ALKBH10B is integrated into abscisic-acid (ABA)-mediated salt-stress signaling. Under salt treatment, loss of ALKBH10B exhibited significantly delayed seed germination. Salt stress could significantly induce the expression of ALKBH10B transcription via the ABA-responsive transcription factor ABI5, which binds directly bind to the ABRE element in the ALKBH10B promoter and activate its transcription. Multi-omic integration of m6A methylomes and transcriptome identified the AP2/ERF transcription factor RAP2.6 as a direct target of ALKBH10B. ALKBH10B removed m6A modifications on RAP2.6 mRNA, accelerating its degradation and modulating the expression of salt-responsive genes. In summary, this study elucidates the salt stress response pathway ABI5-ALKBH10B-RAP2.6 that couples ABA perception to reversible m6A modification, providing mechanistic insight into the intricate regulatory network of dynamic m6A modifications in plant stress adaptation.