Scholarlink

Key message <p><i>VvGA2ox8-Like</i> confers drought tolerance in grape by interacting with <i>VvGRX-S7</i> for inhibited GA biosynthesis and upregulating antioxidant enzyme activities, proline accumulation, and stress-responsive genes.</p> Abstract <p>Drought stress severely limits grape productivity. Gibberellin (GA) metabolism is involved in drought responses, yet the role of specific GA catabolic genes remains unclear. In the present study, <i>VvGA2ox8-Like</i>, a member of the grape GA 2-β-dioxygenase (<i>VvGA2ox</i>) gene family, was identified to confer drought tolerance in Vitis vinifera cv. ‘Pinot Noir’. As a GA catabolic enzyme, <i>VvGA2ox8-Like</i> expression is significantly induced by drought stress. Transient transformation of ‘Pinot Noir’ grape leaves showed that <i>VvGA2ox8-Like</i> overexpression decreased H<sub>2</sub>O<sub>2</sub> and MDA contents, while increasing proline content, antioxidant enzyme activities, and drought-responsive gene expressions; in contrast, virus-induced gene silencing of <i>VvGA2ox8-Like</i> exhibited opposite results. Furthermore, <i>VvGA2ox8-Like</i> overexpression in ‘Pinot Noir’ grape calli further confirmed its positive regulatory role in drought tolerance, whereas CRISPR/Cas9-edited <i>VvGA2ox8-Like</i> calli showed opposite phenotypes. A drought-responsive protein <i>VvGRX-S7</i> was identified as an interacting partner of <i>VvGA2ox8-Like</i> via yeast two-hybrid assay. RNA sequencing analysis revealed that <i>VvGA2ox8-Like</i> overexpression significantly affected the biosynthesis of secondary metabolites, phenylpropanoids, and flavonoids. In conclusion, <i>VvGA2ox8-Like</i> plays a key role in grape drought tolerance, providing theoretical support for elucidating the molecular mechanism of grape drought resistance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

VvGA2ox8-Like from grape confers drought tolerance by activating the antioxidant defense system

  • 期刊论文

  • 发表日期：

    2026年4月24日

查看全文
  • Wenqi Yang,
  • Juanbo Yang,
  • Shixiong Lu,
  • Huimin Gou,
  • Ning Wang,
  • Miao Yuan,
  • Weifeng Ma,
  • Baozhen Zeng,
  • Guoping Liang,
  • Juan Mao

摘要

Key message

VvGA2ox8-Like confers drought tolerance in grape by interacting with VvGRX-S7 for inhibited GA biosynthesis and upregulating antioxidant enzyme activities, proline accumulation, and stress-responsive genes.

Abstract

Drought stress severely limits grape productivity. Gibberellin (GA) metabolism is involved in drought responses, yet the role of specific GA catabolic genes remains unclear. In the present study, VvGA2ox8-Like, a member of the grape GA 2-β-dioxygenase (VvGA2ox) gene family, was identified to confer drought tolerance in Vitis vinifera cv. ‘Pinot Noir’. As a GA catabolic enzyme, VvGA2ox8-Like expression is significantly induced by drought stress. Transient transformation of ‘Pinot Noir’ grape leaves showed that VvGA2ox8-Like overexpression decreased H2O2 and MDA contents, while increasing proline content, antioxidant enzyme activities, and drought-responsive gene expressions; in contrast, virus-induced gene silencing of VvGA2ox8-Like exhibited opposite results. Furthermore, VvGA2ox8-Like overexpression in ‘Pinot Noir’ grape calli further confirmed its positive regulatory role in drought tolerance, whereas CRISPR/Cas9-edited VvGA2ox8-Like calli showed opposite phenotypes. A drought-responsive protein VvGRX-S7 was identified as an interacting partner of VvGA2ox8-Like via yeast two-hybrid assay. RNA sequencing analysis revealed that VvGA2ox8-Like overexpression significantly affected the biosynthesis of secondary metabolites, phenylpropanoids, and flavonoids. In conclusion, VvGA2ox8-Like plays a key role in grape drought tolerance, providing theoretical support for elucidating the molecular mechanism of grape drought resistance.