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Key message <p>The TCP transcription factor PpBRC1 serves as a negative regulator of branching in peach by modulating GA signal transduction gene <i>PpGID1b</i> expression.</p> Abstract <p>Branch number is one of the major determinants in peach (<i>Prunus persica</i> L.) tree architecture. Gibberellins (GAs) regulate various physiological processes, but the roles in regulating peach branching remain unclear. In this study, we found that the main component of GAs in peach was GA<sub>3</sub>, whose content in standard-type peach ‘Okubo’ with high branching was 3.23-fold higher than that in pillar-type peach ‘Zhaoshouhong’ with low branching. Exogenous GA<sub>3</sub> treatment increased branch number and plant height in peach, and the expression of <i>PpGID1b</i> (GA signal transduction gene) was increased up to 37-fold after GA<sub>3</sub> treatment. The promoter activity and expression of <i>PpGID1b</i> in standard-type peach were 2-fold higher than those in pillar-type peach. Yeast one-hybrid, LUC, EMSA, and transient expression in peach axillary bud assays confirmed that PpBRC1 (TCP transcription factor) directly bound to the promoter of <i>PpGID1b</i> and repressed its expression. Furthermore, the expression of <i>PpBRC1</i> was also upregulated by GA<sub>3</sub> treatment, and the expression peak occurred later than that of <i>PpGID1b</i>. Subcellular localization analysis indicated that PpGID1b was located in the cell nucleus, cell membrane, and cell cytoplasm. Transient overexpression and silencing of <i>PpGID1b</i> in peach revealed positive regulation of <i>PpGID1b </i>in&#xa0;branch number, branching rate, and plant height. These findings demonstrated that PpBRC1 inhibited the expression of <i>PpGID1b</i> ‌to modulate‌ GA signal transduction and peach branching, providing a molecular target for labor-saving peach breeding strategies.</p>

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PpBRC1 negatively regulates branching via modulating GA signal transduction gene PpGID1b in peach (Prunus persica)

  • 期刊论文

  • 发表日期：

    2026年5月6日

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  • Yulu Wu,
  • Yajia Zhang,
  • Junjie Zhang,
  • Xiaobei Wang,
  • Haipeng Zhang,
  • Xiaodong Lian,
  • Nan Hou,
  • Xianbo Zheng,
  • Jun Cheng,
  • Wei Wang,
  • Langlang Zhang,
  • Xia Ye,
  • Jidong Li,
  • Jiancan Feng,
  • Lei Wang,
  • Bin Tan

摘要

Key message

The TCP transcription factor PpBRC1 serves as a negative regulator of branching in peach by modulating GA signal transduction gene PpGID1b expression.

Abstract

Branch number is one of the major determinants in peach (Prunus persica L.) tree architecture. Gibberellins (GAs) regulate various physiological processes, but the roles in regulating peach branching remain unclear. In this study, we found that the main component of GAs in peach was GA3, whose content in standard-type peach ‘Okubo’ with high branching was 3.23-fold higher than that in pillar-type peach ‘Zhaoshouhong’ with low branching. Exogenous GA3 treatment increased branch number and plant height in peach, and the expression of PpGID1b (GA signal transduction gene) was increased up to 37-fold after GA3 treatment. The promoter activity and expression of PpGID1b in standard-type peach were 2-fold higher than those in pillar-type peach. Yeast one-hybrid, LUC, EMSA, and transient expression in peach axillary bud assays confirmed that PpBRC1 (TCP transcription factor) directly bound to the promoter of PpGID1b and repressed its expression. Furthermore, the expression of PpBRC1 was also upregulated by GA3 treatment, and the expression peak occurred later than that of PpGID1b. Subcellular localization analysis indicated that PpGID1b was located in the cell nucleus, cell membrane, and cell cytoplasm. Transient overexpression and silencing of PpGID1b in peach revealed positive regulation of PpGID1b in branch number, branching rate, and plant height. These findings demonstrated that PpBRC1 inhibited the expression of PpGID1b ‌to modulate‌ GA signal transduction and peach branching, providing a molecular target for labor-saving peach breeding strategies.