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<p><i>Avena fatua</i> L. is a major grass weed infesting wheat fields worldwide, with the ACCase-inhibiting herbicide fenoxaprop-P-ethyl serving as the primary chemical control agent. However, prolonged and widespread use has resulted in the evolution of resistance in <i>A. fatua</i>, posing a serious threat to effective weed management in wheat production. This study investigates the resistance level and elucidates the underlying mechanisms in a fenoxaprop-P-ethyl-resistant <i>A. fatua</i> population (HZXH-R) collected from China. Whole-plant bioassays demonstrated that the HZXH-R population exhibited a 21.3-fold resistance to fenoxaprop-P-ethyl compared with sensitive population. Sequencing of the ACCase gene detected no known target-site mutations, thereby excluding target-site mutation-mediated resistance. Treatment with cytochrome P450 inhibitors (piperonyl butyl ether [PBO], malathion, and 1-aminobenzotriazole [ABT]) significantly reduced resistance in HZXH-R, implicating P450 monooxygenases in resistance regulation. Transcriptome sequencing identified six genes that were significantly upregulated and two that were downregulated. RT-qPCR validation confirmed that the P450 gene <i>CYP710A8B</i> showed the highest relative expression in the resistant population. This study demonstrates that the <i>A. fatua</i> population HZXH-R exhibits high-level resistance to fenoxaprop-P-ethyl, with the upregulation of <i>CYP710A8B</i> identified as a potential factor in resistance development. These findings provide a foundation for elucidating the evolutionary mechanisms underlying resistance to ACCase-inhibiting herbicides and for guiding the development of more effective, science-based weed management strategies.</p>

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Overexpression of cytochrome P450 CYP710A8B likely contributing resistance to fenoxaprop-P-ethyl in Avena fatua L. from China

  • 期刊论文

  • 发表日期：

    2026年2月5日

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  • Pei Zhang,
  • Yuqing Liu,
  • Xinyang Yuan,
  • Junchuan Song,
  • Runqiang Liu,
  • Hongliang Wang

摘要

Avena fatua L. is a major grass weed infesting wheat fields worldwide, with the ACCase-inhibiting herbicide fenoxaprop-P-ethyl serving as the primary chemical control agent. However, prolonged and widespread use has resulted in the evolution of resistance in A. fatua, posing a serious threat to effective weed management in wheat production. This study investigates the resistance level and elucidates the underlying mechanisms in a fenoxaprop-P-ethyl-resistant A. fatua population (HZXH-R) collected from China. Whole-plant bioassays demonstrated that the HZXH-R population exhibited a 21.3-fold resistance to fenoxaprop-P-ethyl compared with sensitive population. Sequencing of the ACCase gene detected no known target-site mutations, thereby excluding target-site mutation-mediated resistance. Treatment with cytochrome P450 inhibitors (piperonyl butyl ether [PBO], malathion, and 1-aminobenzotriazole [ABT]) significantly reduced resistance in HZXH-R, implicating P450 monooxygenases in resistance regulation. Transcriptome sequencing identified six genes that were significantly upregulated and two that were downregulated. RT-qPCR validation confirmed that the P450 gene CYP710A8B showed the highest relative expression in the resistant population. This study demonstrates that the A. fatua population HZXH-R exhibits high-level resistance to fenoxaprop-P-ethyl, with the upregulation of CYP710A8B identified as a potential factor in resistance development. These findings provide a foundation for elucidating the evolutionary mechanisms underlying resistance to ACCase-inhibiting herbicides and for guiding the development of more effective, science-based weed management strategies.