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Key message <p><i>PmVNS2</i> promotes secondary cell wall formation and enhances xylem development in transgenic poplar.</p> Abstract <p>Secondary cell wall deposition is essential for wood formation in trees, and NAC-domain transcription factors are widely involved in transcriptional networks associated with this process. Here, we identified <i>PmVNS2</i>, a VND-subfamily NAC gene from <i>Pinus massoniana</i>. <i>PmVNS2</i> encodes a conserved NAC-domain protein and shows preferential expression in developing xylem. Heterologous expression of <i>PmVNS2</i> in <i>Populus davidiana</i> × <i>P</i>. <i>bolleana</i> resulted in enhanced plant growth and secondary xylem development, accompanied by increased secondary cell wall thickening. Molecular analyses showed that <i>PmVNS2</i> overexpression was associated with altered expression of genes related to lignin and cellulose biosynthesis. In addition, several downstream transcriptional regulators involved in secondary wall formation were affected, while some hemicellulose-related genes exhibited reduced expression. Together, these findings suggest that <i>PmVNS2</i> may participate in transcriptional regulation associated with secondary cell wall formation and contribute to wood development-related processes in conifer species.</p>

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Heterologous expression of Pinus massoniana VNS2 enhances secondary cell wall deposition in poplar

  • 期刊论文

  • 发表日期：

    2026年1月20日

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  • Yating Tian,
  • Kang Wang,
  • Hao Rong,
  • Meng Xu,
  • Zhouxian Ni,
  • Li-an Xu

摘要

Key message

PmVNS2 promotes secondary cell wall formation and enhances xylem development in transgenic poplar.

Abstract

Secondary cell wall deposition is essential for wood formation in trees, and NAC-domain transcription factors are widely involved in transcriptional networks associated with this process. Here, we identified PmVNS2, a VND-subfamily NAC gene from Pinus massoniana. PmVNS2 encodes a conserved NAC-domain protein and shows preferential expression in developing xylem. Heterologous expression of PmVNS2 in Populus davidiana × P. bolleana resulted in enhanced plant growth and secondary xylem development, accompanied by increased secondary cell wall thickening. Molecular analyses showed that PmVNS2 overexpression was associated with altered expression of genes related to lignin and cellulose biosynthesis. In addition, several downstream transcriptional regulators involved in secondary wall formation were affected, while some hemicellulose-related genes exhibited reduced expression. Together, these findings suggest that PmVNS2 may participate in transcriptional regulation associated with secondary cell wall formation and contribute to wood development-related processes in conifer species.