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Key message <p><i>OsPUB36</i>, a root-preferential E3 ubiquitin ligase, modulates reactive oxygen species homeostasis and regulates rice root development, affecting primary root and root hair growth.</p> Abstract <p>Root development is tightly regulated by spatial gradients of reactive oxygen species (ROS), which coordinate transitions from cell division to elongation and differentiation. Through phylogenetic meta-analysis, we identified a root-preferential subclass of class III <i>PUB</i> E3 ubiquitin ligases (<i>OsPUB31</i>–<i>OsPUB37</i>). Among them, <i>OsPUB36</i> was selected as a representative gene for functional characterization. Overexpressing <i>OsPUB36</i>, an endoplasmic reticulum (ER)-localized protein, resulted in shortened primary roots and elongated root hairs. RNA-seq and proteomic analysis of root hairs from plants overexpressing <i>OsPUB36</i> revealed significant upregulation of class III peroxidases, key enzymes involved in ROS homeostasis. Histochemical staining confirmed enhanced levels of hydrogen peroxide (H₂O₂) in root hair. However, the loss-of-function mutants (<i>ospub36</i> and <i>ospub35 ospub36</i>) displayed no obvious phenotypes, suggesting functional redundancy within these gene subclasses. While yeast two-hybrid screen did not identify direct targets related to ROS or root development, RNA-seq, proteomic, and histochemical analyses suggest that <i>OsPUB36</i> and other class III <i>PUBs</i> modulate ROS homeostasis.</p>

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Root‑preferential OsPUB36 modulates ROS levels and regulates root development

  • 期刊论文

  • 发表日期：

    2025年12月12日

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  • Sunok Moon,
  • Yo-Han Yoo,
  • Ha Eun Park,
  • Hye Jo Min,
  • Ye-Eun Han,
  • Yun Shil Gho,
  • Wonjae Hwang,
  • Jun-Hyup Kim,
  • Cheol Woo Min,
  • Woo-Jong Hong,
  • Woo Taek Kim,
  • Sun Tae Kim,
  • Ki-Hong Jung

摘要

Key message

OsPUB36, a root-preferential E3 ubiquitin ligase, modulates reactive oxygen species homeostasis and regulates rice root development, affecting primary root and root hair growth.

Abstract

Root development is tightly regulated by spatial gradients of reactive oxygen species (ROS), which coordinate transitions from cell division to elongation and differentiation. Through phylogenetic meta-analysis, we identified a root-preferential subclass of class III PUB E3 ubiquitin ligases (OsPUB31–OsPUB37). Among them, OsPUB36 was selected as a representative gene for functional characterization. Overexpressing OsPUB36, an endoplasmic reticulum (ER)-localized protein, resulted in shortened primary roots and elongated root hairs. RNA-seq and proteomic analysis of root hairs from plants overexpressing OsPUB36 revealed significant upregulation of class III peroxidases, key enzymes involved in ROS homeostasis. Histochemical staining confirmed enhanced levels of hydrogen peroxide (H₂O₂) in root hair. However, the loss-of-function mutants (ospub36 and ospub35 ospub36) displayed no obvious phenotypes, suggesting functional redundancy within these gene subclasses. While yeast two-hybrid screen did not identify direct targets related to ROS or root development, RNA-seq, proteomic, and histochemical analyses suggest that OsPUB36 and other class III PUBs modulate ROS homeostasis.