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Key message <p>Constitutive expression of <i>AtYUC1</i> in potato enhances branching and tuber production through upregulation of <i>StARF1</i> and <i>StARF5</i>, which suppress <i>StBRC1a</i> expression.</p> Abstract <p>Branching is a crucial determinant of plant architecture, optimizing light capture, improving environmental adaptability, and enhancing crop yield. Potatoes (<i>Solanum tuberosum</i>) possess above-ground stems and modified stems. To investigate the effect of <i>AtYUC1</i> in these processes, we generated transgenic potato lines constitutively expressing heterologous <i>AtYUC1</i> and performed phenotypic and molecular analyses. Elevated auxin levels in the transgenic lines enhanced branching, plant height, stolon number, and above-ground biomass. The number of tubers was also significantly higher compared to wild-type plants. Molecular analysis revealed significant upregulation of <i>StARF1</i> and <i>StARF5</i>. In addition, <i>StBranched1a</i> (<i>StBRC1a</i>) expression was significantly downregulated in these lines. Auxin treatments further confirmed concentration-dependent modulation of <i>StBRC1a</i> and <i>StBRC1b</i> expression. Further investigations demonstrated that StARF1 and StARF5 bind to the promoter region of <i>StBRC1a</i>, repressing its expression and thereby promoting branching. This study provides valuable insights into the hormonal regulation of branching in potatoes and underscores the potential of genetically manipulating auxin biosynthesis pathways to enhance potato yield.</p>

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Heterologous expression of AtYUC1 enhances potato branching and tuber formation via StARF-mediated repression of StBRC1a

  • 期刊论文

  • 发表日期：

    2025年12月4日

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  • Qingsong Jiao,
  • Hongbao Bai,
  • Longwei Xin,
  • Ting Jia,
  • Xueyun Hu

摘要

Key message

Constitutive expression of AtYUC1 in potato enhances branching and tuber production through upregulation of StARF1 and StARF5, which suppress StBRC1a expression.

Abstract

Branching is a crucial determinant of plant architecture, optimizing light capture, improving environmental adaptability, and enhancing crop yield. Potatoes (Solanum tuberosum) possess above-ground stems and modified stems. To investigate the effect of AtYUC1 in these processes, we generated transgenic potato lines constitutively expressing heterologous AtYUC1 and performed phenotypic and molecular analyses. Elevated auxin levels in the transgenic lines enhanced branching, plant height, stolon number, and above-ground biomass. The number of tubers was also significantly higher compared to wild-type plants. Molecular analysis revealed significant upregulation of StARF1 and StARF5. In addition, StBranched1a (StBRC1a) expression was significantly downregulated in these lines. Auxin treatments further confirmed concentration-dependent modulation of StBRC1a and StBRC1b expression. Further investigations demonstrated that StARF1 and StARF5 bind to the promoter region of StBRC1a, repressing its expression and thereby promoting branching. This study provides valuable insights into the hormonal regulation of branching in potatoes and underscores the potential of genetically manipulating auxin biosynthesis pathways to enhance potato yield.