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Key message <p>The transcription factor <i>CiMYB4</i> enhances the tolerance of <i>Chrysanthemum indicum</i> to cadmium stress by regulating the <i>CiPCS</i> gene and promoting the synthesis of phytochelatins.</p> Abstract <p>Phytochelatins (PCs) are crucial for enhancing plant tolerance and detoxifying heavy metals such as cadmium (Cd). However, the functional roles and regulatory mechanisms of phytochelatin synthase (PCS), the enzyme responsible for PC synthesis in chrysanthemum, remain largely unexplored. In this study, we successfully isolated and cloned a <i>PCS</i> gene from <i>Chrysanthemum indicum</i>, designated as <i>CiPCS</i>. Subcellular localization analysis revealed that CiPCS is localized in both the cytoplasm and nucleus, and Cd stress significantly upregulated its expression. The overexpression of <i>CiPCS</i> enhanced the tolerance of <i>C. indicum</i> to Cd by increasing the activity of antioxidant enzymes and reducing membrane lipid peroxidation. Further analysis indicated that <i>CiPCS</i> is directly regulated by the transcription factor <i>CiMYB4</i>, promoting the synthesis and accumulation of plant chelating peptides (PCs), enhancing the chelation of Cd ions and thereby reducing the toxic effects of Cd on cells. In summary, our study elucidates the regulatory mechanism by which <i>C. indicum</i> responds to Cd stress.</p> Graphic abstract <p></p>

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CiPCS regulated by CiMYB4 enhances the tolerance of Chrysanthemum indicum to cadmium stress

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Shengyan Chen,
  • Liran Yue,
  • Xiaomei Yang,
  • Bin Xia,
  • Hongyao Li,
  • Bin Chen,
  • Yujia Yang,
  • Ying Sun,
  • Qiang Li,
  • Junxin Yan,
  • Miao He

摘要

Key message

The transcription factor CiMYB4 enhances the tolerance of Chrysanthemum indicum to cadmium stress by regulating the CiPCS gene and promoting the synthesis of phytochelatins.

Abstract

Phytochelatins (PCs) are crucial for enhancing plant tolerance and detoxifying heavy metals such as cadmium (Cd). However, the functional roles and regulatory mechanisms of phytochelatin synthase (PCS), the enzyme responsible for PC synthesis in chrysanthemum, remain largely unexplored. In this study, we successfully isolated and cloned a PCS gene from Chrysanthemum indicum, designated as CiPCS. Subcellular localization analysis revealed that CiPCS is localized in both the cytoplasm and nucleus, and Cd stress significantly upregulated its expression. The overexpression of CiPCS enhanced the tolerance of C. indicum to Cd by increasing the activity of antioxidant enzymes and reducing membrane lipid peroxidation. Further analysis indicated that CiPCS is directly regulated by the transcription factor CiMYB4, promoting the synthesis and accumulation of plant chelating peptides (PCs), enhancing the chelation of Cd ions and thereby reducing the toxic effects of Cd on cells. In summary, our study elucidates the regulatory mechanism by which C. indicum responds to Cd stress.

Graphic abstract