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Key message <p>BjZIP1 is a plasma membrane-localized protein. Overexpression of <i>BjZIP1</i> in yeast, <i>Arabidopsis thaliana</i>, and <i>Brassica juncea</i> hairy roots confirmed its role in promoting Cd/Zn uptake.</p> Abstract <p>Heavy metal contamination in agricultural soils significantly threatens global food security. While <i>Brassica juncea</i> is recognized as a promising hyperaccumulator for phytoremediation, the specific transporters mediating its metal uptake remain largely unexplored. Here, we identify BjZIP1, a plasma membrane-localized protein that functions as a transporter&#xa0;for cadmium (Cd) and zinc (Zn) uptake in <i>B. juncea</i>. Heterologous expression of <i>BjZIP1</i> in Cd-sensitive yeast mutant (Δ<i>yap1</i>) increased intracellular accumulation of Cd and Zn by 17.8% and 25.0%, respectively, and consequently enhanced metal sensitivity. In <i>Arabidopsis thaliana</i>, <i>BjZIP1</i> overexpression lines accumulated 31.1–58.3% more Cd and 1.68–2.48-fold higher Zn in roots under metal stress, which was accompanied by a growth inhibition phenotype. Crucially, <i>BjZIP1</i>-overexpressing <i>B. juncea</i> hairy roots accumulated 1.05–1.30-fold more Cd and 1.42–1.92-fold more Zn, alongside a concomitant exacerbation of cellular damage under Cd exposure. Collectively, our results establish <i>BjZIP1</i> as a plasma membrane transporter responsible for Cd and Zn uptake in <i>B. juncea</i>, thereby providing a promising molecular target for genetic enhancement of phytoremediation efficiency.</p> Graphical abstract <p>BjZIP1 functions as a plasma membrane transporter for cadmium and zinc uptake in&#xa0;<i>Brassica juncea</i></p> <p></p>

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BjZIP1, a plasma membrane-localized transporter, mediates cadmium and zinc uptake in Brassica juncea

  • 期刊论文

  • 发表日期：

    2025年11月19日

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  • Yicun Li,
  • Dawei Zhang,
  • Huizi Wen,
  • Jiashi Peng,
  • Jinfeng Wu,
  • Lili Liu,
  • Mingli Yan

摘要

Key message

BjZIP1 is a plasma membrane-localized protein. Overexpression of BjZIP1 in yeast, Arabidopsis thaliana, and Brassica juncea hairy roots confirmed its role in promoting Cd/Zn uptake.

Abstract

Heavy metal contamination in agricultural soils significantly threatens global food security. While Brassica juncea is recognized as a promising hyperaccumulator for phytoremediation, the specific transporters mediating its metal uptake remain largely unexplored. Here, we identify BjZIP1, a plasma membrane-localized protein that functions as a transporter for cadmium (Cd) and zinc (Zn) uptake in B. juncea. Heterologous expression of BjZIP1 in Cd-sensitive yeast mutant (Δyap1) increased intracellular accumulation of Cd and Zn by 17.8% and 25.0%, respectively, and consequently enhanced metal sensitivity. In Arabidopsis thaliana, BjZIP1 overexpression lines accumulated 31.1–58.3% more Cd and 1.68–2.48-fold higher Zn in roots under metal stress, which was accompanied by a growth inhibition phenotype. Crucially, BjZIP1-overexpressing B. juncea hairy roots accumulated 1.05–1.30-fold more Cd and 1.42–1.92-fold more Zn, alongside a concomitant exacerbation of cellular damage under Cd exposure. Collectively, our results establish BjZIP1 as a plasma membrane transporter responsible for Cd and Zn uptake in B. juncea, thereby providing a promising molecular target for genetic enhancement of phytoremediation efficiency.

Graphical abstract

BjZIP1 functions as a plasma membrane transporter for cadmium and zinc uptake in Brassica juncea