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Key message <p>The <i>Triticeae</i> ATG18s are identified, with <i>TaATG18-5</i> suggested to positively regulate FCR resistance via gene silencing&#xa0;and appear to be transcriptionally regulated by TaMYBL1.</p> Abstract <p>As core components in the autophagic pathway, ATG18 family members play critical roles in plant growth, development, and stress resistance. Although ATG18s have been well characterized in multiple species, their functions in wheat, particularly in resistance to fungal diseases, remain unclear. In this study, we systematically identified 76 ATG18 family members from wheat and its diploid/tetraploid relatives. Evolutionary and syntenic analyses revealed that ATG18s expansion in&#xa0;<i>Triticeae</i> species&#xa0;was primarily driven by genome polyploidization, accompanied by tandem gene duplications in specific chromosomal regions (e.g., chromosome 2). <i>TaATG18</i> genes within the same phylogenetic group exhibited similar gene structures and conserved motif distributions. Promoter analysis showed that <i>TaATG18s</i> widely harbored cis-acting elements associated with growth and development, stress responses, hormone signaling, and transcription factor binding (notably MYB-type). Expression profiling indicated that <i>TaATG18s</i> displayed basal expression in various tissues across wheat developmental stages, but were significantly induced by fungal pathogens. Functional studies showed that silencing of <i>TaATG18-5</i> compromised wheat resistance to Fusarium crown rot (FCR), thereby confirming its positive regulatory role in FCR resistance. Furthermore, an MYB-like transcription factor TaMYBL1, identified via yeast one-hybrid (Y1H) screening, directly bound to the cis-element in the <i>TaATG18-5</i> promoter. Expression analysis revealed that <i>TaMYBL1</i> was also induced by&#xa0;<i>Fusarium pseudograminearum</i>, suggesting a potential regulatory module of <i>TaMYBL1</i>-mediated&#xa0;<i>TaATG18-5</i> in wheat FCR resistance. These findings deepen our understanding of the <i>TaATG18</i> gene family and provide <i>TaATG18-5</i> as a potential candidate gene for improving FCR resistance in wheat.</p>

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Identification of ATG18s in Triticeae reveals wheat TaATG18-5′s crucial role in Fusarium crown rot resistance

  • 期刊论文

  • 发表日期：

    2025年10月29日

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  • Meng Zhang,
  • Ming Cheng,
  • Xiaoying Deng,
  • Xiaoxiao Zhang,
  • Guoguo Lv,
  • Yuanyuan Guan,
  • Puwen Song,
  • Yongang Yu,
  • Chunji Liu,
  • Haiyan Hu,
  • Chengwei Li

摘要

Key message

The Triticeae ATG18s are identified, with TaATG18-5 suggested to positively regulate FCR resistance via gene silencing and appear to be transcriptionally regulated by TaMYBL1.

Abstract

As core components in the autophagic pathway, ATG18 family members play critical roles in plant growth, development, and stress resistance. Although ATG18s have been well characterized in multiple species, their functions in wheat, particularly in resistance to fungal diseases, remain unclear. In this study, we systematically identified 76 ATG18 family members from wheat and its diploid/tetraploid relatives. Evolutionary and syntenic analyses revealed that ATG18s expansion in Triticeae species was primarily driven by genome polyploidization, accompanied by tandem gene duplications in specific chromosomal regions (e.g., chromosome 2). TaATG18 genes within the same phylogenetic group exhibited similar gene structures and conserved motif distributions. Promoter analysis showed that TaATG18s widely harbored cis-acting elements associated with growth and development, stress responses, hormone signaling, and transcription factor binding (notably MYB-type). Expression profiling indicated that TaATG18s displayed basal expression in various tissues across wheat developmental stages, but were significantly induced by fungal pathogens. Functional studies showed that silencing of TaATG18-5 compromised wheat resistance to Fusarium crown rot (FCR), thereby confirming its positive regulatory role in FCR resistance. Furthermore, an MYB-like transcription factor TaMYBL1, identified via yeast one-hybrid (Y1H) screening, directly bound to the cis-element in the TaATG18-5 promoter. Expression analysis revealed that TaMYBL1 was also induced by Fusarium pseudograminearum, suggesting a potential regulatory module of TaMYBL1-mediated TaATG18-5 in wheat FCR resistance. These findings deepen our understanding of the TaATG18 gene family and provide TaATG18-5 as a potential candidate gene for improving FCR resistance in wheat.