Scholarlink

Key message <p>This study is the first to demonstrate that <i>Aspergillus niger</i> ZRS14 culture filtrate enhances drought tolerance in Rasheh grapevine by improving antioxidant activity and mitigating oxidative stress under PEG-induced water deficit.</p> Abstract <p>Climate change and water scarcity have made drought stress a major challenge for agriculture and food security. This study investigates the effects of PEG-induced water deficit stress and the culture filtrate of <i>Aspergillus niger</i> strain ZRS14 on the physiological and biochemical traits of Rasheh grape (<i>Vitis vinifera</i> L. cv. Rasheh) under in vitro conditions. A factorial experiment in a completely randomized design was conducted. Water deficit was simulated using polyethylene glycol (PEG) 6000 at 0%, 2%, 4%, and 6% (w/v), while fungal culture filtrate was applied at 48-, 72-, and 120-h post-inoculation, as well as a combined 48 + 120-h treatment. An uninoculated PDB medium (ZRS14 Ctrl) served as the fungal control. Increasing PEG concentrations significantly reduced photosynthetic pigments (chlorophyll a, total chlorophyll, and carotenoids) and elevated oxidative stress markers including hydrogen peroxide (H₂O₂) and malondialdehyde (MDA). In response, antioxidant enzyme activities (CAT, POD, SOD, APX) and total phenolic content (TPC) increased, while total flavonoid content (TFC) showed minimal change. Application of <i>A. niger</i> culture filtrate improved drought tolerance by enhancing pigment levels and antioxidant responses, and by reducing oxidative markers. Under severe drought, TPC levels rose significantly, while TFC remained stable or slightly decreased. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) confirmed the positive role of the fungal filtrate in enhancing biochemical resilience. This is the first study to evaluate the biostimulant potential of <i>A. niger</i> strain ZRS14 for mitigating PEG-induced drought stress in grapevine, offering a promising strategy to improve plant performance under controlled drought conditions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Improving drought stress resilience in grapevine using Aspergillus niger strain ZRS14 under in vitro conditions

  • 期刊论文

  • 发表日期：

    2025年10月17日

查看全文
  • Parisa Moslemi Nezhad Arani,
  • Ali Akbar Mozafari,
  • Morahem Ashengroph,
  • Fatemeh Daneshfar

摘要

Key message

This study is the first to demonstrate that Aspergillus niger ZRS14 culture filtrate enhances drought tolerance in Rasheh grapevine by improving antioxidant activity and mitigating oxidative stress under PEG-induced water deficit.

Abstract

Climate change and water scarcity have made drought stress a major challenge for agriculture and food security. This study investigates the effects of PEG-induced water deficit stress and the culture filtrate of Aspergillus niger strain ZRS14 on the physiological and biochemical traits of Rasheh grape (Vitis vinifera L. cv. Rasheh) under in vitro conditions. A factorial experiment in a completely randomized design was conducted. Water deficit was simulated using polyethylene glycol (PEG) 6000 at 0%, 2%, 4%, and 6% (w/v), while fungal culture filtrate was applied at 48-, 72-, and 120-h post-inoculation, as well as a combined 48 + 120-h treatment. An uninoculated PDB medium (ZRS14 Ctrl) served as the fungal control. Increasing PEG concentrations significantly reduced photosynthetic pigments (chlorophyll a, total chlorophyll, and carotenoids) and elevated oxidative stress markers including hydrogen peroxide (H₂O₂) and malondialdehyde (MDA). In response, antioxidant enzyme activities (CAT, POD, SOD, APX) and total phenolic content (TPC) increased, while total flavonoid content (TFC) showed minimal change. Application of A. niger culture filtrate improved drought tolerance by enhancing pigment levels and antioxidant responses, and by reducing oxidative markers. Under severe drought, TPC levels rose significantly, while TFC remained stable or slightly decreased. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) confirmed the positive role of the fungal filtrate in enhancing biochemical resilience. This is the first study to evaluate the biostimulant potential of A. niger strain ZRS14 for mitigating PEG-induced drought stress in grapevine, offering a promising strategy to improve plant performance under controlled drought conditions.