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Key message <p><i>CpSnRK2.7</i> interacts with CpPP2Cs and enhances salt stress tolerance in <i>Cucurbita pepo</i> by reducing oxidative damage, promoting proline accumulation, and maintaining photosynthetic efficiency.</p> Abstract <p>Sucrose non-fermenting-1-related protein kinase 2 (SnRK2) serves as a pivotal regulator of the ABA signaling cascade and plays an essential role in plant adaptation to abiotic stress. This study elucidated the role of the <i>CpSnRK2.7</i> gene in <i>Cucurbita pepo</i> under salt stress conditions. Overexpression of <i>CpSnRK2.7</i> significantly enhanced salt tolerance by alleviating cellular oxidative damage, as demonstrated by reduced accumulation of MDA, O<sub>2</sub><sup>–</sup>, and H<sub>2</sub>O<sub>2</sub>, while concurrently increasing antioxidant enzyme activities and proline accumulation. Importantly, CpSnRK2.7-overexpressing plants displayed faster stomatal closure and maintained higher photosynthetic efficiency in response to salt stress and ABA treatment. Given that <i>CpSnRK2.7</i> expression is induced by ABA, it is postulated to function within the canonical <i>CpPYL/PYRs–CpPP2Cs–CpSnRK2.7</i> signaling module. Protein–protein interaction analysis via the STRING database and yeast two-hybrid assays revealed direct interactions between <i>CpSnRK2.7</i> and <i>CpPP2C1</i> as well as <i>CpPP2C3</i>. Notably, the interaction between <i>CpPP2C1</i> and multiple <i>CpPYL</i>/<i>PYRs</i> was significantly enhanced following ABA treatment. Taken together, these findings indicate that <i>CpSnRK2.7</i> plays a pivotal role in salt stress adaptation through ABA-mediated signaling and identifies potential targets for enhancing crop tolerance to salinity.</p>

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CpSnRK2.7 interacts with CpPP2Cs to enhance salt tolerance via modulating oxidative stress in Cucurbita pepo

  • 期刊论文

  • 发表日期：

    2025年9月23日

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  • Ke Xu,
  • Ping Wang

摘要

Key message

CpSnRK2.7 interacts with CpPP2Cs and enhances salt stress tolerance in Cucurbita pepo by reducing oxidative damage, promoting proline accumulation, and maintaining photosynthetic efficiency.

Abstract

Sucrose non-fermenting-1-related protein kinase 2 (SnRK2) serves as a pivotal regulator of the ABA signaling cascade and plays an essential role in plant adaptation to abiotic stress. This study elucidated the role of the CpSnRK2.7 gene in Cucurbita pepo under salt stress conditions. Overexpression of CpSnRK2.7 significantly enhanced salt tolerance by alleviating cellular oxidative damage, as demonstrated by reduced accumulation of MDA, O2–, and H2O2, while concurrently increasing antioxidant enzyme activities and proline accumulation. Importantly, CpSnRK2.7-overexpressing plants displayed faster stomatal closure and maintained higher photosynthetic efficiency in response to salt stress and ABA treatment. Given that CpSnRK2.7 expression is induced by ABA, it is postulated to function within the canonical CpPYL/PYRs–CpPP2Cs–CpSnRK2.7 signaling module. Protein–protein interaction analysis via the STRING database and yeast two-hybrid assays revealed direct interactions between CpSnRK2.7 and CpPP2C1 as well as CpPP2C3. Notably, the interaction between CpPP2C1 and multiple CpPYL/PYRs was significantly enhanced following ABA treatment. Taken together, these findings indicate that CpSnRK2.7 plays a pivotal role in salt stress adaptation through ABA-mediated signaling and identifies potential targets for enhancing crop tolerance to salinity.