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Key message <p><b>CsJAZ11 interacts with CsMYC2.2 in the nucleus and positively regulates disease resistance to</b> <Emphasis Type="BoldItalic">Colletotrichum camelliae</Emphasis> <b>in tea plants</b>.</p> Abstract <p>In the jasmonic acid signaling pathway, JASMONATE ZIM DOMAIN (JAZ) proteins act as repressors by inhibiting the activity of MYELOCYTOMATOSIS (MYC) transcription factors. However, the role of JAZ proteins in modulating tea plant resistance to <i>Colletotrichum camelliae</i>, a major causal pathogen of tea anthracnose, remains largely unexplored. In our previous study, yeast two-hybrid analysis revealed an interaction between CsJAZ11 and CsMYC2.2, a transcription factor that negatively regulates tea plant resistance to <i>C. camelliae</i>. In this study, bimolecular fluorescence complementation assays further confirmed that CsJAZ11 interacts with CsMYC2.2 in the nucleus. Co-immunoprecipitation assays also validated this interaction. To investigate the role of CsJAZ11 in regulating tea plant resistance to <i>C. camelliae</i>, transient silencing and overexpression of <i>CsJAZ11</i> were performed in tea plant leaves. Notably, transient silencing of <i>CsJAZ11</i> attenuates disease resistance to <i>C. camelliae</i>, while overexpression of <i>CsJAZ11</i> enhanced disease resistance, suggesting that CsJAZ11 positively regulates tea plant resistance to <i>C. camelliae</i>. Surprisingly, qRT-PCR analysis showed that CsJAZ11 did not inhibit the expression of <i>CsMYC2.2</i>. In contrast, CsJAZ11 positively influenced the expression of <i>CsRPM1</i>, a MeJA-responsive CNL gene essential for disease resistance to <i>C. camelliae</i> in tea plants. Our findings indicate that CsJAZ11 positively regulates tea plant resistance to <i>C. camelliae</i>, potentially through the modulation of <i>CsRPM1</i>, an NBS-LRR-encoding gene. This study provides new insights into the role of JAZ proteins in regulating disease resistance in tea plants.</p>

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CsJAZ11 positively regulates tea plant resistance to Colletotrichum camelliae via transient silencing and overexpression

  • 期刊论文

  • 发表日期：

    2025年7月13日

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  • Yuhan Fan,
  • Yuchun Wang,
  • Yiyi Tu,
  • Hong Jiang,
  • Ting Xu,
  • Chaoling Wei,
  • Wuyun Lv

摘要

Key message

CsJAZ11 interacts with CsMYC2.2 in the nucleus and positively regulates disease resistance to Colletotrichum camelliae in tea plants.

Abstract

In the jasmonic acid signaling pathway, JASMONATE ZIM DOMAIN (JAZ) proteins act as repressors by inhibiting the activity of MYELOCYTOMATOSIS (MYC) transcription factors. However, the role of JAZ proteins in modulating tea plant resistance to Colletotrichum camelliae, a major causal pathogen of tea anthracnose, remains largely unexplored. In our previous study, yeast two-hybrid analysis revealed an interaction between CsJAZ11 and CsMYC2.2, a transcription factor that negatively regulates tea plant resistance to C. camelliae. In this study, bimolecular fluorescence complementation assays further confirmed that CsJAZ11 interacts with CsMYC2.2 in the nucleus. Co-immunoprecipitation assays also validated this interaction. To investigate the role of CsJAZ11 in regulating tea plant resistance to C. camelliae, transient silencing and overexpression of CsJAZ11 were performed in tea plant leaves. Notably, transient silencing of CsJAZ11 attenuates disease resistance to C. camelliae, while overexpression of CsJAZ11 enhanced disease resistance, suggesting that CsJAZ11 positively regulates tea plant resistance to C. camelliae. Surprisingly, qRT-PCR analysis showed that CsJAZ11 did not inhibit the expression of CsMYC2.2. In contrast, CsJAZ11 positively influenced the expression of CsRPM1, a MeJA-responsive CNL gene essential for disease resistance to C. camelliae in tea plants. Our findings indicate that CsJAZ11 positively regulates tea plant resistance to C. camelliae, potentially through the modulation of CsRPM1, an NBS-LRR-encoding gene. This study provides new insights into the role of JAZ proteins in regulating disease resistance in tea plants.