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Key message <p>Genome-wide identified 144 MYB family members in <i>B. papyrifera</i>. Integrated correlation analysis and target gene-binding motif prediction indicate that <i>BpMYB135</i> is vital in regulating selenium metabolism.</p> Abstract <p>Selenium is an essential micronutrient for maintaining the health of humans and animals. <i>Broussonetia papyrifera</i>, a forage tree with high nutritional value, exhibits a remarkable ability to accumulate selenium. Although previous studies have preliminarily unfolded the molecular mechanisms underlying selenium accumulation, the roles of transcription factors in regulating selenium uptake and transformation remain poorly understood. This study used various strategies including bioinformatic, physiological, and molecular experiments to explore candidates regarding Se metabolism. Briefly, 144 MYB transcription factor family members were identified and classified into four types (R1, R2R3, R1R2R3, and R4), with phylogenetic analysis further dividing them into 58 subfamilies. The promoters of those <i>BpMYBs</i> contain numerous <i>cis</i>-acting elements associated with plant growth, development, and stress response. qRT-PCR assay confirmed 8 of 15 <i>BpMYB</i>s exhibit a remarkable correlation with selenium content at the threshold absolute value of 0.5. Additionally, foliar application of exogenous abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA) reveals different response patterns of <i>BpMYBs</i>. The subcellular localization assay simultaneously verifies that the candidate BpMYB135 functions within the nucleus. Overall, this funding highlights the potential regulatory mechanisms of selenium metabolism in <i>B. papyrifera</i>, providing a foundation for improving its forage value through genetic modification.</p>

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Genome-wide identification of MYB gene family and exploration of selenium metabolism-related candidates in paper mulberry (Broussonetia papyrifera)

  • 期刊论文

  • 发表日期：

    2025年3月25日

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  • Changye Zhu,
  • Qiangwen Chen,
  • Longfei Guo,
  • Shiming Deng,
  • Weiwei Zhang,
  • Shuiyuan Cheng,
  • Xin Cong,
  • Feng Xu

摘要

Key message

Genome-wide identified 144 MYB family members in B. papyrifera. Integrated correlation analysis and target gene-binding motif prediction indicate that BpMYB135 is vital in regulating selenium metabolism.

Abstract

Selenium is an essential micronutrient for maintaining the health of humans and animals. Broussonetia papyrifera, a forage tree with high nutritional value, exhibits a remarkable ability to accumulate selenium. Although previous studies have preliminarily unfolded the molecular mechanisms underlying selenium accumulation, the roles of transcription factors in regulating selenium uptake and transformation remain poorly understood. This study used various strategies including bioinformatic, physiological, and molecular experiments to explore candidates regarding Se metabolism. Briefly, 144 MYB transcription factor family members were identified and classified into four types (R1, R2R3, R1R2R3, and R4), with phylogenetic analysis further dividing them into 58 subfamilies. The promoters of those BpMYBs contain numerous cis-acting elements associated with plant growth, development, and stress response. qRT-PCR assay confirmed 8 of 15 BpMYBs exhibit a remarkable correlation with selenium content at the threshold absolute value of 0.5. Additionally, foliar application of exogenous abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA) reveals different response patterns of BpMYBs. The subcellular localization assay simultaneously verifies that the candidate BpMYB135 functions within the nucleus. Overall, this funding highlights the potential regulatory mechanisms of selenium metabolism in B. papyrifera, providing a foundation for improving its forage value through genetic modification.