Scholarlink

<p>Carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) is an emerging threat to healthcare settings in many countries, principally in South Asia. The current study was aimed to identify, evaluate whole-genome and characterize the prophages in genome of CRAB strain, recovered from patients of Lahore General Hospital, Lahore. More than 200 samples were collected and identified by morphological and biochemical tests. These strains were also subjected to a comprehensive antimicrobial susceptibility evaluation using Kirby-Bauer method and further confirmed as CRAB strains by exploring <i>bla</i><sub>OXA−51</sub>. In addition, the whole-genome evaluation of a <i>Acinetobacter baumannii</i> UOL-KIMZ-24-2 was carried out using various Bioinformatics tools. A total of 150 strains of <i>A. baumannii</i> were recovered and identified in the current study. Among them, 49% strains were found resistant to carbapenem. The <i>bla</i><sub>OXA<i>−51</i></sub> was found prevalent in the genome of <i>A. baumannii</i> recovered from medical ICU (38%). In addition, the UOL-KIMZ-24-2 genome analysis based on multilocus sequence typing (MLST) highlighted that UOL-KIMZ-24-2 belonged to ST2 (Pasteur scheme) sequence type. A total of 29 antimicrobial resistance (AMR) genes were present, importantly, <i>bla</i><sub>OXA−66</sub>, <i>bla</i><sub>OXA−23</sub> and <i>bla</i><sub>OXA−25</sub>. The mobile genetic elements (MGEs) were identified as transposases and belonged to four classes e.g. <i>IS15d1</i>,<i> ISAba24</i>,<i> ISEc29</i>, and <i>ISEc35</i>. A total of 14 virulence factors encoded by 58 different genes were detected in UOL-KIMZ-24-2. In addition, the phage sequences were identified in genome of UOL-KIMZ-24-2, divided into 3 regions. In conclusion, UOL-KIMZ-24-2 contained a mixture of AMR genes, MGEs. prophages sequences and virulence genes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Whole-genome evaluation and prophages characterization associated with genome of carbapenem-resistant Acinetobacter baumannii UOL-KIMZ-24-2

  • 期刊论文

  • 发表日期：

    2025年1月24日

查看全文
  • Kiran Fatima,
  • Syed Zeeshan Haider Naqvi,
  • Hazrat Ali,
  • Noor Hassan,
  • Farheen Ansari,
  • Sidrah Saleem,
  • Shah Jahan,
  • Mushtaq Ahmad,
  • Aniqa Nawaz,
  • Anam Saqib

摘要

Carbapenem-resistant Acinetobacter baumannii (CRAB) is an emerging threat to healthcare settings in many countries, principally in South Asia. The current study was aimed to identify, evaluate whole-genome and characterize the prophages in genome of CRAB strain, recovered from patients of Lahore General Hospital, Lahore. More than 200 samples were collected and identified by morphological and biochemical tests. These strains were also subjected to a comprehensive antimicrobial susceptibility evaluation using Kirby-Bauer method and further confirmed as CRAB strains by exploring blaOXA−51. In addition, the whole-genome evaluation of a Acinetobacter baumannii UOL-KIMZ-24-2 was carried out using various Bioinformatics tools. A total of 150 strains of A. baumannii were recovered and identified in the current study. Among them, 49% strains were found resistant to carbapenem. The blaOXA−51 was found prevalent in the genome of A. baumannii recovered from medical ICU (38%). In addition, the UOL-KIMZ-24-2 genome analysis based on multilocus sequence typing (MLST) highlighted that UOL-KIMZ-24-2 belonged to ST2 (Pasteur scheme) sequence type. A total of 29 antimicrobial resistance (AMR) genes were present, importantly, blaOXA−66, blaOXA−23 and blaOXA−25. The mobile genetic elements (MGEs) were identified as transposases and belonged to four classes e.g. IS15d1, ISAba24, ISEc29, and ISEc35. A total of 14 virulence factors encoded by 58 different genes were detected in UOL-KIMZ-24-2. In addition, the phage sequences were identified in genome of UOL-KIMZ-24-2, divided into 3 regions. In conclusion, UOL-KIMZ-24-2 contained a mixture of AMR genes, MGEs. prophages sequences and virulence genes.