Background <p>In 2020, the Organised Early Cancer Diagnosis Guideline (eKFE-RL) programme was introduced in Germany. Women up to the age of 34 years are entitled to annual cytology-based screening. Women aged 35 years and over are entitled to a combined test (cytology and HPV) every 3 years. An age-related assessment algorithm determines the procedure if there are abnormal findings.</p> Method <p>A total of 222,851 data were evaluated and analysed from the first round of screening in 2020–2021 according to the oKFE-RL: the prevalence of HPV, its age distribution in general and in HPV-positive findings (<i>n</i> = 10,405), as well as the distribution of HPV 16, 18 and other HPV types, are shown in cervical intraepithelial neoplasia (CIN) lesions. CIN 3 lesions are depicted and compared with the baseline value for 2019.</p> Results <p>A threefold increase in CIN 3 compared with 2019 was determined. The peak age for CIN 3 is in women aged 35–39 years. The HPV-positive rate is 8.11 %.</p>