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<p>This study presents the fabrication and evaluation of electrospun polycaprolactone (PCL)-based scaffolds embedded with a green-synthesized halloysite nanoclay–silver nanoparticle (HNC/Ag) hybrid for bone tissue engineering applications. The HNC/Ag nanocomposites were successfully synthesized and integrated into the PCL matrix to produce bioactive nanofibrous scaffolds. Morphological (SEM/TEM), structural (XRD, FTIR), and mechanical analyses confirmed the formation of a dual-phase crystalline nanostructure with enhanced hydrophilicity and tensile extension. Antibacterial testing revealed significant inhibition of both <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> in PLC/HNC/Ag-treated scaffolds, compared to PCL and PCL/HNC controls. Adipose-derived mesenchymal stem cells (ADMSCs) exhibited superior attachment, proliferation, and osteogenic differentiation on PCL/HNC/Ag scaffolds. Enhanced expression of <i>RUNX2</i>, <i>OCN</i>, and <i>ColA1</i>, along with elevated ALP activity and calcium mineralization, indicated robust osteoinductive capacity. These multifunctional scaffolds demonstrate excellent biocompatibility, antimicrobial efficacy, and bone-regenerative potential, making them promising candidates for future clinical applications.</p>

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Biofunctional electrospun PCL scaffolds incorporating halloysite nanoclay–silver nanohybrids for antibacterial and osteoinductive bone regeneration

  • 期刊论文

  • 发表日期：

    2025年12月23日

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  • Parisa kashanizadeh,
  • Iman Rad,
  • Shadie hatamie,
  • Elaheh Esmaeili

摘要

This study presents the fabrication and evaluation of electrospun polycaprolactone (PCL)-based scaffolds embedded with a green-synthesized halloysite nanoclay–silver nanoparticle (HNC/Ag) hybrid for bone tissue engineering applications. The HNC/Ag nanocomposites were successfully synthesized and integrated into the PCL matrix to produce bioactive nanofibrous scaffolds. Morphological (SEM/TEM), structural (XRD, FTIR), and mechanical analyses confirmed the formation of a dual-phase crystalline nanostructure with enhanced hydrophilicity and tensile extension. Antibacterial testing revealed significant inhibition of both Staphylococcus aureus and Escherichia coli in PLC/HNC/Ag-treated scaffolds, compared to PCL and PCL/HNC controls. Adipose-derived mesenchymal stem cells (ADMSCs) exhibited superior attachment, proliferation, and osteogenic differentiation on PCL/HNC/Ag scaffolds. Enhanced expression of RUNX2, OCN, and ColA1, along with elevated ALP activity and calcium mineralization, indicated robust osteoinductive capacity. These multifunctional scaffolds demonstrate excellent biocompatibility, antimicrobial efficacy, and bone-regenerative potential, making them promising candidates for future clinical applications.