Scholarlink

<p>Putrescine is a naturally occurring biogenic amine synthesized primarily through the decarboxylation of ornithine. This low-molecular-weight aliphatic diamine possesses high thermal stability and chemical resistance, making it an important monomer for polyamide synthesis, particularly in the production of nylon 4,6. Conventional petrochemical routes for putrescine synthesis, based on halogenation of succinonitrile derived from acrylonitrile, are energy-intensive and environmentally hazardous. Consequently, the development of bio-based and sustainable alternatives has captivated a great deal of attention. This review summarizes recent advances in engineering microbial catalysts for putrescine production, highlighting their biological functions, industrial relevance, and the strategies applied to enhance microbial synthesis. Special emphasis is placed on metabolic and pathway engineering, transporter optimization, and host design in Escherichia coli and Corynebacterium glutamicum. The review also discusses utilization of renewable feedstocks and emerging synthetic biology tools, including CRISPR/Cas 9-based genome editing technologies. In addition, current methods for product recovery, purification, and analytical quantification are outlined. Overall, this work integrates current knowledge and future directions in rational biocatalyst design for sustainable and scalable putrescine biomanufacturing.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Engineering microbial catalysts for putrescine production: a gateway towards renewable routes for green polyamides

  • 期刊论文

  • 发表日期：

    2025年12月4日

查看全文
  • Keerthi Sasikumar,
  • K. Madhavan Nampoothiri

摘要

Putrescine is a naturally occurring biogenic amine synthesized primarily through the decarboxylation of ornithine. This low-molecular-weight aliphatic diamine possesses high thermal stability and chemical resistance, making it an important monomer for polyamide synthesis, particularly in the production of nylon 4,6. Conventional petrochemical routes for putrescine synthesis, based on halogenation of succinonitrile derived from acrylonitrile, are energy-intensive and environmentally hazardous. Consequently, the development of bio-based and sustainable alternatives has captivated a great deal of attention. This review summarizes recent advances in engineering microbial catalysts for putrescine production, highlighting their biological functions, industrial relevance, and the strategies applied to enhance microbial synthesis. Special emphasis is placed on metabolic and pathway engineering, transporter optimization, and host design in Escherichia coli and Corynebacterium glutamicum. The review also discusses utilization of renewable feedstocks and emerging synthetic biology tools, including CRISPR/Cas 9-based genome editing technologies. In addition, current methods for product recovery, purification, and analytical quantification are outlined. Overall, this work integrates current knowledge and future directions in rational biocatalyst design for sustainable and scalable putrescine biomanufacturing.