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<p>This research’s aim includes the encapsulation of chrysin (CHR) and piperine (PIP) in chitosan (CS) and lecithin nanoparticles (CHR-PIP-LCS-NPs), and the assessment of their therapeutic activity. The biosynthesized particles underwent thorough physicochemical characterization using a number of methods, including DLS, FTIR, and FESEM. Their effect on the cell viability of A2780, MCF-7, PANC cancer cells, and HFF normal cells was measured by cell counting with MTT Assay, which is a standard assay, and was undertaken to evaluate the cytotoxicity of these particles. The potential pro-apoptotic and pro-inflammatory effects of CHR-PIP-LCS-NPs were investigated through the qPCR method. Also, the antioxidant activity of these NPs was studied using the ABTS and DPPH assays. CHR-PIP-LCS-NPs exhibited spherical, uniform (PDI = 0.21), and stable (+ 30.45&#xa0;mV) dispersion with average diameters of 214.59&#xa0;nm. The encapsulation efficiency of PIP and CHR was reported as 90.5% and 79.2%, respectively. This system predominantly reduced the proliferation of cancer cells in the PANC cell lines (IC<sub>50</sub> = 14&#xa0;mg/mL), but it did not significantly change the HFF cells (IC<sub>50</sub> &gt; 500&#xa0;mg/mL), indicating that it is selectively toxic. Upregulation of caspase 3, 8, and 9 genes confirmed the pro-apoptotic activity of CHR-PIP-LCS-NPs. Cell cycle analysis and fluorescence staining further supported apoptotic induction potential. In addition, CHR-PIP-LCS-NPs demonstrated the ability to induce inflammation by increasing the expression of the interleukin-6 gene. Moreover, these NPs exhibited free radical scavenging activity against ABTS (IC<sub>50</sub>: 125&#xa0;µg/mL) and DPPH (IC<sub>50</sub>: 1&#xa0;mg/mL). Based on these experimental results, LCS-NPs loaded with PIP and CHR are promising anticancer candidates for cancer therapy.</p>

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Synergistic anticancer, inflammatory, and antioxidant effect of piperine and chrysin combination loaded on lipid-polymer hybrid nanoparticles

  • 期刊论文

  • 发表日期：

    2025年9月1日

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  • Parisa Pourali,
  • Jebur Sahib Abed Alowaidi,
  • Parisa Beyramabdi,
  • Ghazal Hosseini Torshiz,
  • Tina Taslim,
  • Shirin Ghahramani,
  • Masoud Homayouni Tabrizi

摘要

This research’s aim includes the encapsulation of chrysin (CHR) and piperine (PIP) in chitosan (CS) and lecithin nanoparticles (CHR-PIP-LCS-NPs), and the assessment of their therapeutic activity. The biosynthesized particles underwent thorough physicochemical characterization using a number of methods, including DLS, FTIR, and FESEM. Their effect on the cell viability of A2780, MCF-7, PANC cancer cells, and HFF normal cells was measured by cell counting with MTT Assay, which is a standard assay, and was undertaken to evaluate the cytotoxicity of these particles. The potential pro-apoptotic and pro-inflammatory effects of CHR-PIP-LCS-NPs were investigated through the qPCR method. Also, the antioxidant activity of these NPs was studied using the ABTS and DPPH assays. CHR-PIP-LCS-NPs exhibited spherical, uniform (PDI = 0.21), and stable (+ 30.45 mV) dispersion with average diameters of 214.59 nm. The encapsulation efficiency of PIP and CHR was reported as 90.5% and 79.2%, respectively. This system predominantly reduced the proliferation of cancer cells in the PANC cell lines (IC50 = 14 mg/mL), but it did not significantly change the HFF cells (IC50 > 500 mg/mL), indicating that it is selectively toxic. Upregulation of caspase 3, 8, and 9 genes confirmed the pro-apoptotic activity of CHR-PIP-LCS-NPs. Cell cycle analysis and fluorescence staining further supported apoptotic induction potential. In addition, CHR-PIP-LCS-NPs demonstrated the ability to induce inflammation by increasing the expression of the interleukin-6 gene. Moreover, these NPs exhibited free radical scavenging activity against ABTS (IC50: 125 µg/mL) and DPPH (IC50: 1 mg/mL). Based on these experimental results, LCS-NPs loaded with PIP and CHR are promising anticancer candidates for cancer therapy.