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<p>Cartilage tissue regeneration is a significant challenge in orthopedics, especially with the aging population. This study introduces a novel co-electrospun scaffold composed of polycaprolactone (PCL), gelatin, chitosan, and carbon nanotubes (CNT) for cartilage tissue engineering. The scaffold was doped with varying CNT concentrations to optimize its properties. In-vitro tests were performed to assess scaffold morphology, chemical composition, water-scaffold interaction, mechanical properties, and cell viability. Scaffolds with 0.1 and 0.2% CNT (0.2 CNT) showed homogenous morphology, while the 0.4% CNT resulted in non-homogeneity. The 0.2 CNT scaffold demonstrated hydrophilicity (64.5° contact angle), adequate swelling, favorable biodegradation, and suitable mechanical properties for cartilage regeneration. The 0.2 CNT supported cell adhesion and cell viability, and no dead cells were detected in the live/dead test. Additionally, the 0.2 CNT scaffold showed 95.5 ± 1.5% cell viability after 7 days in MTT assay. Additionally, it demonstrated chondrogenic differentiation capability according to alcian blue staining results. Therefore, the 0.2 CNT scaffold is a promising candidate for cartilage tissue engineering and is proposed for further in-vivo investigation.</p>

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In-vitro study of co-electrospun PCL/gelatin/chitosan scaffolds enhanced with carbon nanotube for cartilage regeneration

  • 期刊论文

  • 发表日期：

    2025年5月26日

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  • Mohammad Irani,
  • Mohammad Hossein Nasirtabrizi,
  • Elham Ezzatzadeh,
  • Ali Sadeghianmaryan

摘要

Cartilage tissue regeneration is a significant challenge in orthopedics, especially with the aging population. This study introduces a novel co-electrospun scaffold composed of polycaprolactone (PCL), gelatin, chitosan, and carbon nanotubes (CNT) for cartilage tissue engineering. The scaffold was doped with varying CNT concentrations to optimize its properties. In-vitro tests were performed to assess scaffold morphology, chemical composition, water-scaffold interaction, mechanical properties, and cell viability. Scaffolds with 0.1 and 0.2% CNT (0.2 CNT) showed homogenous morphology, while the 0.4% CNT resulted in non-homogeneity. The 0.2 CNT scaffold demonstrated hydrophilicity (64.5° contact angle), adequate swelling, favorable biodegradation, and suitable mechanical properties for cartilage regeneration. The 0.2 CNT supported cell adhesion and cell viability, and no dead cells were detected in the live/dead test. Additionally, the 0.2 CNT scaffold showed 95.5 ± 1.5% cell viability after 7 days in MTT assay. Additionally, it demonstrated chondrogenic differentiation capability according to alcian blue staining results. Therefore, the 0.2 CNT scaffold is a promising candidate for cartilage tissue engineering and is proposed for further in-vivo investigation.