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<p>This paper proposes a novel and highly sensitive acetylcholinesterase (AChE) enzyme-based biosensor for organophosphate pesticide (OPs) detection using biosynthesized cerium oxide (CeO<sub>2</sub>)-incorporated polyaniline (PANI) matrix as a sensing platform. The properties of CeO<sub>2</sub> and PANI effectively enhance the performance of the developed biosensor. The excellent bio-affinity of PANI toward the AChE enzyme enhances the degree of immobilization. Moreover, the redox nature and strong catalyzing power toward the phosphorylation reaction of CeO<sub>2</sub> enhance the electron kinetics. The fabricated biosensor shows high sensitivity of 0.59&#xa0;μA (pM)<sup>−1</sup> (cm)<sup>−2</sup> and a low detection limit (LOD) of 0.65&#xa0;pM for OPs (paraoxon-ethyl (PE)) detection. The accuracy and availability of the proposed biosensor for PE detection were studied in two real samples (carrot and soil). The result demonstrates high recovery rates (100.5–107%) and good accuracy (RSD &lt; 5%) in all spiked samples. These findings highlight the biosensor’s potential for real-world applications, confirming its effectiveness and reliability for sensitive pesticide detection in complex matrices.</p>

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CeO₂ nanocrystal-enhanced polyaniline biosensor for organophosphate detection: a step toward sustainable agriculture

  • 期刊论文

  • 发表日期：

    2025年5月24日

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  • Saroj Paneru,
  • Sweety,
  • D. Kumar

摘要

This paper proposes a novel and highly sensitive acetylcholinesterase (AChE) enzyme-based biosensor for organophosphate pesticide (OPs) detection using biosynthesized cerium oxide (CeO2)-incorporated polyaniline (PANI) matrix as a sensing platform. The properties of CeO2 and PANI effectively enhance the performance of the developed biosensor. The excellent bio-affinity of PANI toward the AChE enzyme enhances the degree of immobilization. Moreover, the redox nature and strong catalyzing power toward the phosphorylation reaction of CeO2 enhance the electron kinetics. The fabricated biosensor shows high sensitivity of 0.59 μA (pM)−1 (cm)−2 and a low detection limit (LOD) of 0.65 pM for OPs (paraoxon-ethyl (PE)) detection. The accuracy and availability of the proposed biosensor for PE detection were studied in two real samples (carrot and soil). The result demonstrates high recovery rates (100.5–107%) and good accuracy (RSD < 5%) in all spiked samples. These findings highlight the biosensor’s potential for real-world applications, confirming its effectiveness and reliability for sensitive pesticide detection in complex matrices.