Scholarlink

<p>In this study, we report the production of flower-shaped HRP-Cu<sup>2+</sup> hybrid nano biocatalyst (HRP-Cu<sup>2+</sup> HNF) from the complexation between horseradish peroxidase (HRP) enzyme and Cu<sup>2+</sup> ions, and investigate catalytic activity and stability of the obtained nanoflowers on the polymerization of some vinyl monomers (styrene, methylmethacrylate, acrylamide and <i>N</i>-isopropylacrylamide). Polymerizations of these monomers, except water soluble acrylamide, were accomplished under emulsion conditions using cationic, anionic and non-ionic surfactants in the presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and 2,4-pentanedione mediator. Optimum polymerizations were achieved under the conditions of non-ionic surfactant (tween 40) used. HRP-Cu<sup>2+</sup> HNF mediated polymerizations resulted in very high yields and molecular weights (<i>M</i><sub>n</sub>) of the polymers. Optimum polymerization of styrene with 84% of yield (<i>M</i><sub>n</sub> = 319&#xa0;kDa) was accomplished at room temperature. However, the highest polymerization yields for acrylamide (96%, <i>M</i><sub>n</sub> = 171&#xa0;kDa) and <i>N</i>-isopropylacrylamide (85%, <i>M</i><sub>n</sub> = 185&#xa0;kDa) was achieved at 70&#xa0;°C. Similarly, optimum polymerization of methylmethacrylate was accomplished with 84% of yield (<i>M</i><sub>n</sub> = 190&#xa0;kDa) at 60&#xa0;°C. While free-HRP loses its catalytic activity at 60&#xa0;°C and above temperatures, HRP-Cu<sup>2+</sup> HNF showed very high catalytic activity and stability even at 70&#xa0;°C. Increasing activity and stability of hybrid nanoflowers provide significant advantages for both scientific and industrial applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Investigation of the free-radical polymerization of vinyl monomers using horseradish peroxidase (HRP) nanoflowers

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月24日

查看全文
  • Gulbahar Ozaydin,
  • Muge Mirioglu,
  • Seyma Dadi,
  • Ismail Ocsoy,
  • Ersen Gokturk

摘要

In this study, we report the production of flower-shaped HRP-Cu2+ hybrid nano biocatalyst (HRP-Cu2+ HNF) from the complexation between horseradish peroxidase (HRP) enzyme and Cu2+ ions, and investigate catalytic activity and stability of the obtained nanoflowers on the polymerization of some vinyl monomers (styrene, methylmethacrylate, acrylamide and N-isopropylacrylamide). Polymerizations of these monomers, except water soluble acrylamide, were accomplished under emulsion conditions using cationic, anionic and non-ionic surfactants in the presence of hydrogen peroxide (H2O2) and 2,4-pentanedione mediator. Optimum polymerizations were achieved under the conditions of non-ionic surfactant (tween 40) used. HRP-Cu2+ HNF mediated polymerizations resulted in very high yields and molecular weights (Mn) of the polymers. Optimum polymerization of styrene with 84% of yield (Mn = 319 kDa) was accomplished at room temperature. However, the highest polymerization yields for acrylamide (96%, Mn = 171 kDa) and N-isopropylacrylamide (85%, Mn = 185 kDa) was achieved at 70 °C. Similarly, optimum polymerization of methylmethacrylate was accomplished with 84% of yield (Mn = 190 kDa) at 60 °C. While free-HRP loses its catalytic activity at 60 °C and above temperatures, HRP-Cu2+ HNF showed very high catalytic activity and stability even at 70 °C. Increasing activity and stability of hybrid nanoflowers provide significant advantages for both scientific and industrial applications.