Scholarlink

<p>This study examines a nanosuspension of secondary metabolites (NSM) derived from <i>Streptomyces chrestomyceticus</i> strain ADP4, previously reported for its antifungal efficacy against Candida spp., and extends its evaluation to WHO-listed high-priority bacterial pathogens. The antibacterial potential of the NSM was assessed against <i>Staphylococcus aureus</i> ATCC 25,923, <i>Enterococcus faecium</i> ATCC 49,924, and <i>Enterococcus faecalis</i> ATCC 29,212. Transmission electron microscopy revealed the formation of uniformly dispersed, spherical nanoparticles with an average diameter of 14 ± 4.26&#xa0;nm. Compared with the crude secondary metabolites (SMs), the NSM demonstrated noticeably enhanced antibacterial performance, reflected by increased zones of inhibition (10%, 16%, and 26%), significant reductions in minimum inhibitory concentrations (53%, 42%, and 71%), and improved minimum biofilm inhibitory concentrations (41%, 30%, and 70%) against <i>S. aureus</i>, <i>E. faecium</i>, and <i>E. faecalis</i>, respectively. The observed enhancement in antimicrobial and antibiofilm activity highlights the role of nano-formulation in improving the bioavailability and biological efficiency of microbial SMs. Also, the flow cytometric analysis of bacterial cells treated with SMs and NSM revealed an increase in membrane permeability in a dose- and time-dependent manner. These findings highlight the potential of actinobacteria-derived NSM as environmentally relevant and support its applicability in macromolecular and surface-associated biomedical applications, including antibiofilm coatings for medical devices, wound dressings, and infection-resistant biomaterials making NSM as a potential strategy for sustainable and microbe-driven innovation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluation of Antibacterial Properties of Nanosuspension of Secondary Metabolites from Streptomyces chrestomyceticus ADP4

  • 期刊论文

  • 发表日期：

    2026年8月3日

查看全文
  • Shelly Singh,
  • Shilpa Sharma,
  • Ashok K. Dubey

摘要

This study examines a nanosuspension of secondary metabolites (NSM) derived from Streptomyces chrestomyceticus strain ADP4, previously reported for its antifungal efficacy against Candida spp., and extends its evaluation to WHO-listed high-priority bacterial pathogens. The antibacterial potential of the NSM was assessed against Staphylococcus aureus ATCC 25,923, Enterococcus faecium ATCC 49,924, and Enterococcus faecalis ATCC 29,212. Transmission electron microscopy revealed the formation of uniformly dispersed, spherical nanoparticles with an average diameter of 14 ± 4.26 nm. Compared with the crude secondary metabolites (SMs), the NSM demonstrated noticeably enhanced antibacterial performance, reflected by increased zones of inhibition (10%, 16%, and 26%), significant reductions in minimum inhibitory concentrations (53%, 42%, and 71%), and improved minimum biofilm inhibitory concentrations (41%, 30%, and 70%) against S. aureus, E. faecium, and E. faecalis, respectively. The observed enhancement in antimicrobial and antibiofilm activity highlights the role of nano-formulation in improving the bioavailability and biological efficiency of microbial SMs. Also, the flow cytometric analysis of bacterial cells treated with SMs and NSM revealed an increase in membrane permeability in a dose- and time-dependent manner. These findings highlight the potential of actinobacteria-derived NSM as environmentally relevant and support its applicability in macromolecular and surface-associated biomedical applications, including antibiofilm coatings for medical devices, wound dressings, and infection-resistant biomaterials making NSM as a potential strategy for sustainable and microbe-driven innovation.