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<p>Probiotics have emerged as promising modulators of bone metabolism. This study evaluated the bone-protective effects of <i>Streptococcus thermophilus</i> Yilife-SST01 alone or in combination with <i>Bifidobacterium bifidum</i> BL-99 using MC3T3-E1 osteoblasts and a hindlimb unloading-induced osteoporosis model in Sprague-Dawley rats. <i>S. thermophilus</i> Yilife-SST01 promoted osteoblast mineralization and significantly inhibited osteoclast activity in both cancellous and cortical bone. Micro-CT analysis demonstrated marked improvements in bone microarchitecture, with bone mineral density increasing by 38.70% in the SST01 group and by 78.30% in the combined treatment group. Probiotic supplementation also increased fecal short-chain fatty acids, particularly butyrate and propionate, and reduced unloading-induced inflammatory cytokines, including TNF-α, IL-6, IL-1β, and IL-10. Whole-genome analysis confirmed the safety of both probiotic strains, with no classical virulence or acquired antimicrobial resistance genes detected. These findings indicate that <i>S. thermophilus</i> Yilife-SST01, especially when combined with <i>B. bifidum</i> BL-99, effectively alleviates unloading-induced osteoporosis by promoting bone formation, suppressing bone resorption, enhancing SCFA production, and modulating inflammatory responses, supporting its potential as a probiotic strategy for improving bone health.</p>

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Combined Supplementation with Streptococcus Thermophilus Yilife-SST 01 and Bifidobacterium Bifidum BL-99 Mitigates Unloading-Induced Osteoporosis in Rats

  • 期刊论文

  • 发表日期：

    2026年7月25日

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  • Lingtong Guo,
  • Baolei Li,
  • Shuo Liu,
  • Ruizi Wang,
  • Jian He,
  • Philip A. Wescombe,
  • Yanmei Sun,
  • Baochao Hou,
  • Shiwei Wang

摘要

Probiotics have emerged as promising modulators of bone metabolism. This study evaluated the bone-protective effects of Streptococcus thermophilus Yilife-SST01 alone or in combination with Bifidobacterium bifidum BL-99 using MC3T3-E1 osteoblasts and a hindlimb unloading-induced osteoporosis model in Sprague-Dawley rats. S. thermophilus Yilife-SST01 promoted osteoblast mineralization and significantly inhibited osteoclast activity in both cancellous and cortical bone. Micro-CT analysis demonstrated marked improvements in bone microarchitecture, with bone mineral density increasing by 38.70% in the SST01 group and by 78.30% in the combined treatment group. Probiotic supplementation also increased fecal short-chain fatty acids, particularly butyrate and propionate, and reduced unloading-induced inflammatory cytokines, including TNF-α, IL-6, IL-1β, and IL-10. Whole-genome analysis confirmed the safety of both probiotic strains, with no classical virulence or acquired antimicrobial resistance genes detected. These findings indicate that S. thermophilus Yilife-SST01, especially when combined with B. bifidum BL-99, effectively alleviates unloading-induced osteoporosis by promoting bone formation, suppressing bone resorption, enhancing SCFA production, and modulating inflammatory responses, supporting its potential as a probiotic strategy for improving bone health.