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<p>In this study, genes encoding components of the type VI (T6SS) secretion system and putative effectors were searched in rhizobial strains belonging to the <i>Rhizobium leguminosarum</i> species complex. For this purpose, the genome assembly of the new <i>Rhizobium laguerreae</i> Wp19 strain (genospecies N) isolated from wild pea nodules was done and compared to the type strains. Comparative analysis of <i>tss</i> gene clusters was conducted in the new Wp19 strain and other <i>R. laguerreae</i> strains, as well as <i>R. ruizarguesonis</i> and <i>R. johnstonii</i> strains with available genomes. The resulting genome assemblies of <i>R. laguerreae</i> Wp19 and other strains were analyzed to compare the homology of genes that encode the T6SS proteins — <i>tssI</i> (VrgG) and <i>tssD</i> (Hcp). A detailed study of their genomic environment revealed a number of genes that encode putative effector proteins. Among the identified proteins, some are conserved, such as proteins with the DUF2169 (adaptor), and DUF4150 domains, while others are found only in individual strains (e.g., proteins consisting of 358/360 and 309/310 amino acids in <i>R. johnstonii</i> 3841, <i>R. ruizarguesonis</i> UPM1133, and <i>R. beringeri</i> SM51). In contrast, the effector protein consisting of 197 amino acids was found in almost all <i>R. laguerreae</i> strains, which may be related to its specific function in these strains.</p>

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Comparative Genomic Analysis of the Type VI Secretion System in Strains of the Rhizobium leguminosarum Species Complex with Variable Host Specificity

  • 期刊论文

  • 发表日期：

    2026年7月15日

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  • Olga A. Pavlova,
  • Alexandra V. Dolgikh,
  • Alexandra A. Shalyakina,
  • Elizaveta A. Salnikova,
  • Alina M. Dymo,
  • Tatyana S. Aksenova,
  • Oksana N. Kurchak,
  • Olga P. Onishchuk,
  • Elena A. Dolgikh

摘要

In this study, genes encoding components of the type VI (T6SS) secretion system and putative effectors were searched in rhizobial strains belonging to the Rhizobium leguminosarum species complex. For this purpose, the genome assembly of the new Rhizobium laguerreae Wp19 strain (genospecies N) isolated from wild pea nodules was done and compared to the type strains. Comparative analysis of tss gene clusters was conducted in the new Wp19 strain and other R. laguerreae strains, as well as R. ruizarguesonis and R. johnstonii strains with available genomes. The resulting genome assemblies of R. laguerreae Wp19 and other strains were analyzed to compare the homology of genes that encode the T6SS proteins — tssI (VrgG) and tssD (Hcp). A detailed study of their genomic environment revealed a number of genes that encode putative effector proteins. Among the identified proteins, some are conserved, such as proteins with the DUF2169 (adaptor), and DUF4150 domains, while others are found only in individual strains (e.g., proteins consisting of 358/360 and 309/310 amino acids in R. johnstonii 3841, R. ruizarguesonis UPM1133, and R. beringeri SM51). In contrast, the effector protein consisting of 197 amino acids was found in almost all R. laguerreae strains, which may be related to its specific function in these strains.