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<p>A novel bacterial strain, designated MF1-13<sup>ᵀ</sup>, was isolated from tidal-flat sediment collected in Gochang, Republic of Korea. Cells of strain MF1-13<sup>ᵀ</sup> are Gram-stain-negative, facultatively anaerobic, motile, and short rod-shaped to ovoid, forming pale yellow colonies on marine agar. The strain produces catalase and oxidase. It grows at 25–30&#xa0;°C (optimum, 30&#xa0;°C), at pH 6–8.0 (optimum, pH 7.0), and in the presence of 0–5% (w/v) NaCl, with optimal growth at 2% (w/v). Phylogenetic analysis based on 16&#xa0;S rRNA and genome sequences placed strain MF1-13<sup>ᵀ</sup> within the family <i>Roseobacteraceae</i> (class <i>Alphaproteobacteria</i>), showing highest sequence similarity to <i>Pseudooceanicola nitratireducens</i> JLT1210<sup>ᵀ</sup> (97.2%), <i>Pseudooceanicola onchidii</i> XY-99<sup>ᵀ</sup> (96.8%) and <i>Pseudooceanicola flagellatus</i> DY470<sup>ᵀ</sup> (97.2%). The major polar lipids are phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The genome of strain MF1-13<sup>ᵀ</sup> is 4.24 Mbp in size, with a G + C content of 61.2%. The strain hydrolyzes starch, gelatin, and Tween 20, 40, and 80, but do not hydrolyze casein or DNase. The predominant cellular fatty acids are C<sub>16:0</sub>, C<sub>18:1</sub> <i>ω</i>7<i>c</i> 11-methyl, and Summed Feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i>/C<sub>18:1</sub> <i>ω</i>6<i>c</i>). Genomic comparisons (ANI, AAI, and dDDH) between strain MF1-13ᵀ and related taxa were well below species delineation thresholds, supporting its classification as a novel species. Based on polyphasic taxonomic analysis, strain MF1-13<sup>ᴛ</sup> represents a novel species of the genus <i>Pseudooceanicola</i>, for which the name <i>Pseudooceanicola aestuariicola</i> sp. nov. is proposed. The type strain is MF1-13<sup>ᵀ</sup> (= KEMB 21563<sup>ᵀ</sup> = KCTC 8775<sup>ᵀ</sup> = JCM 37675<sup>ᵀ</sup>).</p>

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Isolation and Characterization of Pseudooceanicola aestuariicola sp. nov., from Tidal-Flat Sediments

  • 期刊论文

  • 发表日期：

    2026年5月13日

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  • Ayesha Rehman,
  • Shahzadi Raheela Anum,
  • Naureen Khalid,
  • Keun-Hyeok Yang,
  • Suk Chan Lee,
  • Sathiyaraj Srivansan,
  • Sang Seob Lee

摘要

A novel bacterial strain, designated MF1-13ᵀ, was isolated from tidal-flat sediment collected in Gochang, Republic of Korea. Cells of strain MF1-13ᵀ are Gram-stain-negative, facultatively anaerobic, motile, and short rod-shaped to ovoid, forming pale yellow colonies on marine agar. The strain produces catalase and oxidase. It grows at 25–30 °C (optimum, 30 °C), at pH 6–8.0 (optimum, pH 7.0), and in the presence of 0–5% (w/v) NaCl, with optimal growth at 2% (w/v). Phylogenetic analysis based on 16 S rRNA and genome sequences placed strain MF1-13ᵀ within the family Roseobacteraceae (class Alphaproteobacteria), showing highest sequence similarity to Pseudooceanicola nitratireducens JLT1210ᵀ (97.2%), Pseudooceanicola onchidii XY-99ᵀ (96.8%) and Pseudooceanicola flagellatus DY470ᵀ (97.2%). The major polar lipids are phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The genome of strain MF1-13ᵀ is 4.24 Mbp in size, with a G + C content of 61.2%. The strain hydrolyzes starch, gelatin, and Tween 20, 40, and 80, but do not hydrolyze casein or DNase. The predominant cellular fatty acids are C16:0, C18:1 ω7c 11-methyl, and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c). Genomic comparisons (ANI, AAI, and dDDH) between strain MF1-13ᵀ and related taxa were well below species delineation thresholds, supporting its classification as a novel species. Based on polyphasic taxonomic analysis, strain MF1-13ᴛ represents a novel species of the genus Pseudooceanicola, for which the name Pseudooceanicola aestuariicola sp. nov. is proposed. The type strain is MF1-13ᵀ (= KEMB 21563ᵀ = KCTC 8775ᵀ = JCM 37675ᵀ).