<p>To clarify the genomic information of strain <i>Botrytis euroamericana</i> HZ-011 and to screen the relevant functional genes for weed control. The whole genome of the DNA of strain HZ-011 was sequenced in this study using the third-generation single-molecule sequencing technology. The splicing and assembly of the three generations of sequencing data were performed using Hifiasm software, the gene assembly results were predicted using GeneMarkS software, and the predicted gene protein sequences were compared with the four major databases for gene annotation using the BLASTTx program. The results showed that the total gene length of strain HZ-011 was 4,402,230&#xa0;bp, with 41.72% GC content. In total, 12,654 coding genes and 385 non-coding genes were predicted for this strain by whole genome sequencing. The basic annotation results showed that 12,250 genes were annotated, accounting for 96.88% of the total number of coding genes. The advanced genome annotation results showed that 1,629 genes were annotated in the CAZy database, and 1,864 genes were annotated in the PHI database, of which 54 genes were associated with the pathogenicity of pathogenic bacteria. In addition, 59 clusters of secondary metabolite synthesis genes and 12,654 signaling proteins were predicted, of which 1,088 secreted proteins were predicted, accounting for 8.6% of the total number of predicted signal peptide proteins. The whole-genome data of strain HZ-011 screened a large number of genes related to cell wall degradation enzymes and secondary metabolite synthesis regulation. The screened naphthalene gene cluster may represent a new pathogenic substance and is worthy of further in-depth research. The genome-wide data of strain HZ-011 provide a good foundation for in-depth studies of the pathogenicity-regulating genes of this strain and for investigating the pathogenicity mechanism of this strain.</p>