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<p>Diosgenin, a steroid with multiple pharmacological properties such as anti-cancerous, anti-microbial, anti-inflammatory, cardioprotective, etc., is primarily derived from <i>Dioscorea</i> species, and represents an important component of steroidal medications. Due to its economic importance and rising demand, identifying sustainable alternative sources of diosgenin is imperative. Endophytes, a group of plant-associated microbial communities inhabiting the internal tissues of plants, have been reported to produce a wide range of bioactive compounds, including host metabolites. The study is aimed at bioprospecting of bacterial endophytes from <i>Dioscorea bulbifera</i> (air potato), an underutilized tuber crop collected from Chhattisgarh for the production of diosgenin. Eleven bacterial endophytes were isolated from the bulbs of <i>D. bulbifera</i>, and based on 16S rRNA gene sequencing, were identified as belonging to five genera: <i>Enterobacter</i>, <i>Pseudomonas</i>, <i>Bacillus</i>, <i>Priestia</i>, and <i>Staphylococcus</i>. Subsequently, a preliminary screening of their crude extracts for diosgenin production was performed using Fourier transform infrared spectroscopy (FTIR). The <i>Pseudomonas aeruginosa</i> D3 crude extract was found to have FTIR spectra similar to that of standard diosgenin. The endophyte-mediated diosgenin production was further confirmed by High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) analysis. This is the first report of diosgenin production by a bacterial endophyte of <i>D. bulbifera</i>, and highlights the potential of exploring endophytes as a promising alternative source for plant metabolites.</p>

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Synthesis of Diosgenin by Pseudomonas aeruginosa D3, an Endophyte of Dioscorea bulbifera

  • 期刊论文

  • 发表日期：

    2026年4月28日

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  • Krishna Nanda Dhal,
  • Pramod Kumar Sahu,
  • Amrita Gupta,
  • Adarsh Kumar,
  • Zaryab Shafi,
  • Parul Johri,
  • Sushma Mishra,
  • Alok Kumar Srivastava

摘要

Diosgenin, a steroid with multiple pharmacological properties such as anti-cancerous, anti-microbial, anti-inflammatory, cardioprotective, etc., is primarily derived from Dioscorea species, and represents an important component of steroidal medications. Due to its economic importance and rising demand, identifying sustainable alternative sources of diosgenin is imperative. Endophytes, a group of plant-associated microbial communities inhabiting the internal tissues of plants, have been reported to produce a wide range of bioactive compounds, including host metabolites. The study is aimed at bioprospecting of bacterial endophytes from Dioscorea bulbifera (air potato), an underutilized tuber crop collected from Chhattisgarh for the production of diosgenin. Eleven bacterial endophytes were isolated from the bulbs of D. bulbifera, and based on 16S rRNA gene sequencing, were identified as belonging to five genera: Enterobacter, Pseudomonas, Bacillus, Priestia, and Staphylococcus. Subsequently, a preliminary screening of their crude extracts for diosgenin production was performed using Fourier transform infrared spectroscopy (FTIR). The Pseudomonas aeruginosa D3 crude extract was found to have FTIR spectra similar to that of standard diosgenin. The endophyte-mediated diosgenin production was further confirmed by High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) analysis. This is the first report of diosgenin production by a bacterial endophyte of D. bulbifera, and highlights the potential of exploring endophytes as a promising alternative source for plant metabolites.