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<p>A Gram-stain-positive, rod-shaped, non-motile bacterium, designated strain MI7-26<sup>T</sup>, with organic phosphate solubilizing ability was isolated from rhizosphere soil of <i>Meconopsis integrifolia</i>, Nyingchi, Xizang, China. Optimal growth conditions were observed at 30 ℃, pH 7.0 and 0.5% (w/v) NaCl. A comparative analysis of 16&#xa0;S rRNA gene sequences revealed that strain MI7-26<sup>T</sup> showed the highest similarity to <i>Arthrobacter bambusae</i> JCM 19,335<sup>T</sup> (98.6% sequence similarity); <i>Arthrobacter gyeryongensis</i> DCY72<sup>T</sup> (98.5%); <i>Pseudarthrobacter enclensis</i> NIO-1008<sup>T</sup> (97.5%). The genome size was 4.81&#xa0;Mb and the G + C content was 63.1%. Whole genome-based analysis revealed that strain MI7-26<sup>T</sup> is more closely related to <i>Arthrobacter ramosus</i>, <i>Arthrobacter bambusae</i>, and <i>Arthrobacter gyeryongensis</i>, with both the Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values below the thresholds used for species delineation (ANI &lt; 90%, dDDH &lt; 40%). The major fatty acids were anteiso-C<sub>15:0</sub> and anteiso-C<sub>17:0</sub>. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids, one unidentified aminolipid and two unidentified glycolipids. The major quinone was menaquinone MK-9(H<sub>2</sub>). The cell-wall peptidoglycan was A3<i>α</i> type, containing lysine and alanine. Phenotypically, strain MI7-26<sup>T</sup> differed from its close relatives by being positive for the Voges-Proskauer reaction, methyl red test, and hydrolysis of Tween 80, as well as for enzyme activities of <i>β</i>-glucuronidase and N-acetyl-<i>β</i>-glucosaminidase. On the basis of phenotypic, phylogenetic and genotypic data, strain MI7-26<sup>T</sup> is considered to represent a novel species of the genus <i>Arthrobacter</i>, for which the name <i>Arthrobacter rhizophilus</i> sp. nov. is proposed. The type strain is MI7-26<sup>T</sup> (= GDMCC 1.3254<sup>T</sup> = JCM 35693<sup>T</sup>).</p>

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Arthrobacter rhizophilus sp. nov., an Organic Phosphate Solubilizing Bacterium Isolated from Rhizosphere Soil of Meconopsis integrifolia

  • 期刊论文

  • 发表日期：

    2026年4月5日

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  • Yongxia Wang,
  • Chengxin Zhang,
  • Yan Wang,
  • Delong Kong,
  • Zhen Xing,
  • Zhiyong Ruan

摘要

A Gram-stain-positive, rod-shaped, non-motile bacterium, designated strain MI7-26T, with organic phosphate solubilizing ability was isolated from rhizosphere soil of Meconopsis integrifolia, Nyingchi, Xizang, China. Optimal growth conditions were observed at 30 ℃, pH 7.0 and 0.5% (w/v) NaCl. A comparative analysis of 16 S rRNA gene sequences revealed that strain MI7-26T showed the highest similarity to Arthrobacter bambusae JCM 19,335T (98.6% sequence similarity); Arthrobacter gyeryongensis DCY72T (98.5%); Pseudarthrobacter enclensis NIO-1008T (97.5%). The genome size was 4.81 Mb and the G + C content was 63.1%. Whole genome-based analysis revealed that strain MI7-26T is more closely related to Arthrobacter ramosus, Arthrobacter bambusae, and Arthrobacter gyeryongensis, with both the Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH) values below the thresholds used for species delineation (ANI < 90%, dDDH < 40%). The major fatty acids were anteiso-C15:0 and anteiso-C17:0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids, one unidentified aminolipid and two unidentified glycolipids. The major quinone was menaquinone MK-9(H2). The cell-wall peptidoglycan was A3α type, containing lysine and alanine. Phenotypically, strain MI7-26T differed from its close relatives by being positive for the Voges-Proskauer reaction, methyl red test, and hydrolysis of Tween 80, as well as for enzyme activities of β-glucuronidase and N-acetyl-β-glucosaminidase. On the basis of phenotypic, phylogenetic and genotypic data, strain MI7-26T is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter rhizophilus sp. nov. is proposed. The type strain is MI7-26T (= GDMCC 1.3254T = JCM 35693T).