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<p><i>Klebsiella pneumoniae</i> is an ESKAPE pathogen, it is implicated in various opportunistic infections. This study was aimed to isolate and characterize SBP phage against <i>Klebsiella pneumoniae</i> K-112 serotype. SBP phage was isolated from sewage water and exhibits serotype specific narrow host range. Its burst size is 198 pfu/cell with 25&#xa0;min of latent period. SBP phage showed excellent growth controlling ability for 8–10&#xa0;h against planktonic cells. It was able to remove 24&#xa0;h old biofilm. Host bacterial strains showed lower bacteriophage insensitive mutant frequency against SBP phage corresponding to 2.42 ± 0.49 × 10<sup>− 7</sup> at MOI-1. SBP phage demonstrated excellent stability at various conditions of pH and temperature. It has 49&#xa0;kb double stranded DNA genome with GC content of 48.7%. A total of 78 ORFs were predicted, among them 22 (28%) encoded proteins with known function. SBP phage carries DNA-directed-DNA and RNA polymerase genes. Replication and transcription-related genes, DNA packaging enzymes were annotated in its genome. No lysogeny related genes were identified. It contains p-21 like SAR-endolysin lysis cassette. It was classified as lytic virus of <i>Jedunavirus</i> genus of <i>Caudoviricetes</i> class. It does not contain any bacteria related virulence factors and antibiotic resistance genes, which makes it a suitable candidate for phage therapy.</p>

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Antimicrobial Potential and Genomic Insights of Novel Klebsiella Phage SBP against K-112 Serotype

  • 期刊论文

  • 发表日期：

    2026年4月3日

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  • Muhammad Asif,
  • Iqbal Ahmad Alvi,
  • Sibgha,
  • Muhammad Luqman Qadir,
  • Shafiq-ur-Rehman

摘要

Klebsiella pneumoniae is an ESKAPE pathogen, it is implicated in various opportunistic infections. This study was aimed to isolate and characterize SBP phage against Klebsiella pneumoniae K-112 serotype. SBP phage was isolated from sewage water and exhibits serotype specific narrow host range. Its burst size is 198 pfu/cell with 25 min of latent period. SBP phage showed excellent growth controlling ability for 8–10 h against planktonic cells. It was able to remove 24 h old biofilm. Host bacterial strains showed lower bacteriophage insensitive mutant frequency against SBP phage corresponding to 2.42 ± 0.49 × 10− 7 at MOI-1. SBP phage demonstrated excellent stability at various conditions of pH and temperature. It has 49 kb double stranded DNA genome with GC content of 48.7%. A total of 78 ORFs were predicted, among them 22 (28%) encoded proteins with known function. SBP phage carries DNA-directed-DNA and RNA polymerase genes. Replication and transcription-related genes, DNA packaging enzymes were annotated in its genome. No lysogeny related genes were identified. It contains p-21 like SAR-endolysin lysis cassette. It was classified as lytic virus of Jedunavirus genus of Caudoviricetes class. It does not contain any bacteria related virulence factors and antibiotic resistance genes, which makes it a suitable candidate for phage therapy.