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<p>A bisphenol A degrading actinobacterium, strain A6<sup>T</sup>, was isolated from a waste digestion system in Chaozhou, China. Cells of strain A6<sup>T</sup> were Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped, and forming yellow colonies on Reasoner’s 2&#xa0;A agar. Phylogenetic analyses based on the 16&#xa0;S rRNA gene showed that strain A6<sup>T</sup> shared 96.8% sequence similarity with <i>Enemella dayhoffiae</i> NML 130,396<sup>T</sup> and formed a distinct lineage in the family <i>Propionibacteriaceae</i> in the phylogenetic trees. Genome-based phylogenetic analyses indicated that strain A6<sup>T</sup> could be assigned as a novel genus in the family <i>Propionibacteriaceae</i>. The cellular components, including anteiso-C<sub>15:0</sub> (40.0%) as a predominant fatty acid, MK-9(H4) as the major respiratory quinone and a DNA G + C content of 67.2% supported strain A6<sup>T</sup> as a member of the family <i>Propionibacteriaceae</i>. However, strain A6<sup>T</sup> also contained C<sub>11:0</sub>-3-OH (14.4%), iso-C<sub>16:0</sub> (11.3%), and C<sub>11:0</sub> (10.6%) as predominant fatty acids which distinguished it from other genera in the family <i>Propionibacteriaceae</i>. In addition, strain A6<sup>T</sup> was negative in enzyme activity of esterase (C4), esterase lipase (C8), aminase of leucine, and negative in the utilization of most carbon sources, which were different from its close related genera in the family <i>Propionibacteriaceae</i> and supported it as a novel genus. Therefore, the name <i>Pseudenemella bisphenolivorans</i> gen. nov., sp. nov. is proposed. The type strain is A6<sup>T</sup> (= GDMCC 1.3237<sup>T</sup> =KCTC 92291<sup>T</sup>).</p>

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Pseudenemella bisphenolivorans gen. nov., sp. nov., a Bisphenol A Degrading Actinobacterium Isolated from Waste Digestion System

  • 期刊论文

  • 发表日期：

    2026年2月11日

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  • Kangni Ren,
  • Chuansheng Geng,
  • Qinghua Zhang,
  • Tongchu Deng,
  • Meiying Xu

摘要

A bisphenol A degrading actinobacterium, strain A6T, was isolated from a waste digestion system in Chaozhou, China. Cells of strain A6T were Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped, and forming yellow colonies on Reasoner’s 2 A agar. Phylogenetic analyses based on the 16 S rRNA gene showed that strain A6T shared 96.8% sequence similarity with Enemella dayhoffiae NML 130,396T and formed a distinct lineage in the family Propionibacteriaceae in the phylogenetic trees. Genome-based phylogenetic analyses indicated that strain A6T could be assigned as a novel genus in the family Propionibacteriaceae. The cellular components, including anteiso-C15:0 (40.0%) as a predominant fatty acid, MK-9(H4) as the major respiratory quinone and a DNA G + C content of 67.2% supported strain A6T as a member of the family Propionibacteriaceae. However, strain A6T also contained C11:0-3-OH (14.4%), iso-C16:0 (11.3%), and C11:0 (10.6%) as predominant fatty acids which distinguished it from other genera in the family Propionibacteriaceae. In addition, strain A6T was negative in enzyme activity of esterase (C4), esterase lipase (C8), aminase of leucine, and negative in the utilization of most carbon sources, which were different from its close related genera in the family Propionibacteriaceae and supported it as a novel genus. Therefore, the name Pseudenemella bisphenolivorans gen. nov., sp. nov. is proposed. The type strain is A6T (= GDMCC 1.3237T =KCTC 92291T).