<p>A N,N-dimethylformamide-degrading bacterium, strain HB0163ᵀ, was isolated from activated sludge of a wastewater treatment plant in Hangzhou, Zhejiang Province, China. Cells are Gram-stain-negative, aerobic, oxidase-positive, non-motile rods (0.4–0.6 × 1.0–1.5 μm). Growth occurred at 20–45&#xa0;°C (optimum 35&#xa0;°C), pH 5.0–9.5 (optimum 7.5–8.0), and with 0–6.0% (w/v) NaCl (optimum 2.0–3.0%). Chemotaxonomic analysis showed Ubiquinone-10 as the respiratory quinone, C<sub>19:0</sub> cyclo ω8c, C<sub>18:1</sub> ω7c and C<sub>16:0</sub> as major fatty acids, and a polar lipid profile consisting of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unknown phosphoglycolipids, three unknown aminolipids, two unknown phospholipids, and one unknown lipid. The genomic DNA G + C content was 50.7%. Phylogenetic analysis of the 16S rRNA gene indicated its placement within the genus&#xa0;<i>Pseudochrobactrum</i>. Average nucleotide identity and digital DNA–DNA hybridization values between HB0163ᵀ and its closest relatives were 80.4–81.1% (&lt; 95%) and 22.7–23.4% (&lt; 70%), respectively, below the established species thresholds. Based on the polyphasic characterization, strain HB0163ᵀ represents a novel species of the genus&#xa0;<i>Pseudochrobactrum</i>, for which the name&#xa0;<i>Pseudochrobactrum albidum</i>&#xa0;sp. nov. is proposed. The type strain is HB0163ᵀ (= MCCC 1K09177ᵀ = JCM 37008ᵀ).</p>

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Pseudochrobactrum albidum sp. nov., a N,N-dimethylformamide-Degrading Bacterium Isolated from Activated Sludge of a Wastewater Treatment Plant

  • Qiyun Zheng,
  • Wenwu Zhang,
  • Yue Su,
  • Yusi Yang,
  • Xiaoyu Bai,
  • Hongzhe Hu,
  • Shasha Qiu,
  • Xinyi Huang,
  • Tao Wang,
  • Can Chen

摘要

A N,N-dimethylformamide-degrading bacterium, strain HB0163ᵀ, was isolated from activated sludge of a wastewater treatment plant in Hangzhou, Zhejiang Province, China. Cells are Gram-stain-negative, aerobic, oxidase-positive, non-motile rods (0.4–0.6 × 1.0–1.5 μm). Growth occurred at 20–45 °C (optimum 35 °C), pH 5.0–9.5 (optimum 7.5–8.0), and with 0–6.0% (w/v) NaCl (optimum 2.0–3.0%). Chemotaxonomic analysis showed Ubiquinone-10 as the respiratory quinone, C19:0 cyclo ω8c, C18:1 ω7c and C16:0 as major fatty acids, and a polar lipid profile consisting of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unknown phosphoglycolipids, three unknown aminolipids, two unknown phospholipids, and one unknown lipid. The genomic DNA G + C content was 50.7%. Phylogenetic analysis of the 16S rRNA gene indicated its placement within the genus Pseudochrobactrum. Average nucleotide identity and digital DNA–DNA hybridization values between HB0163ᵀ and its closest relatives were 80.4–81.1% (< 95%) and 22.7–23.4% (< 70%), respectively, below the established species thresholds. Based on the polyphasic characterization, strain HB0163ᵀ represents a novel species of the genus Pseudochrobactrum, for which the name Pseudochrobactrum albidum sp. nov. is proposed. The type strain is HB0163ᵀ (= MCCC 1K09177ᵀ = JCM 37008ᵀ).