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<p>A novel aerobic, Gram-stain-positive, non-motile, non-spore-forming, short rod-shaped strain X10-3<sup>T</sup>, was isolated from Daihai Lake, Inner Mongolia, China. The strain formed circular, smooth, transparent, orange-colored colonies with convex elevation and entire margins, which measured approximately 1.0–4.0&#xa0;mm in diameter after 48&#xa0;h of incubation on Luria-Bertani agar at 32&#xa0;°C. The strain was able to grow at pH levels between 6.5 and 9.0 (optimal at 7.5-8.0), temperatures from 4 to 40&#xa0;°C (optimal at 37&#xa0;°C), and in 0–13% (optimal at 2–3%, w/v) NaCl. The complete genome of strain X10-3<sup>T</sup> comprises 3,244,194&#xa0;bp with a DNA G + C content of 45.9%. Phylogenetic analysis revealed the closest relationship to members of the genus <i>Planococcus</i>, showing 16S rRNA gene sequence similarities of 99.2% with <i>P. salinarum</i> DSM 23820<sup>T</sup>, 98.8% with <i>P. wigleyi</i> Sa1BUA13<sup>T</sup>, 98.6% with <i>P. koreense</i> JG07<sup>T</sup>, and 98.6% with <i>P. halotolerans</i> SCU63<sup>T</sup>. The average nucleotide identity between strain X10-3<sup>T</sup> and the species within the genus <i>Planococcus</i> fell below the species delineation thresholds of 95%, and the digital DNA-DNA hybridization values were lower than 70%. Chemotaxonomic analysis revealed that the predominant cellular fatty acids of strain X10-3<sup>T</sup> were anteiso-C<sub>15:0</sub> and C<sub>16:1</sub> ω7c alcohol. The primary cellular polar lipids included phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, while the predominant isoprenoid quinones were menaquinone-8 and menaquinone-7. Based on these comprehensive analyses, strain X10-3<sup>T</sup> represents a new species of the genus <i>Planococcus</i>, for which the name <i>Planococcus daihaiensis</i> sp. nov. is proposed. The type strain is X10-3<sup>T</sup> (= CGMCC 1.60163<sup>T</sup> = KCTC 43688<sup>T</sup>).</p>

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Planococcus Daihaiensis sp. nov. Isolated from the Lake Water in Daihai

  • 期刊论文

  • 发表日期：

    2026年2月6日

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  • Gen Che,
  • Kai Jiang,
  • Chunling Cao,
  • Lijia Ye,
  • Yanxing Wang,
  • Yu Hong

摘要

A novel aerobic, Gram-stain-positive, non-motile, non-spore-forming, short rod-shaped strain X10-3T, was isolated from Daihai Lake, Inner Mongolia, China. The strain formed circular, smooth, transparent, orange-colored colonies with convex elevation and entire margins, which measured approximately 1.0–4.0 mm in diameter after 48 h of incubation on Luria-Bertani agar at 32 °C. The strain was able to grow at pH levels between 6.5 and 9.0 (optimal at 7.5-8.0), temperatures from 4 to 40 °C (optimal at 37 °C), and in 0–13% (optimal at 2–3%, w/v) NaCl. The complete genome of strain X10-3T comprises 3,244,194 bp with a DNA G + C content of 45.9%. Phylogenetic analysis revealed the closest relationship to members of the genus Planococcus, showing 16S rRNA gene sequence similarities of 99.2% with P. salinarum DSM 23820T, 98.8% with P. wigleyi Sa1BUA13T, 98.6% with P. koreense JG07T, and 98.6% with P. halotolerans SCU63T. The average nucleotide identity between strain X10-3T and the species within the genus Planococcus fell below the species delineation thresholds of 95%, and the digital DNA-DNA hybridization values were lower than 70%. Chemotaxonomic analysis revealed that the predominant cellular fatty acids of strain X10-3T were anteiso-C15:0 and C16:1 ω7c alcohol. The primary cellular polar lipids included phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, while the predominant isoprenoid quinones were menaquinone-8 and menaquinone-7. Based on these comprehensive analyses, strain X10-3T represents a new species of the genus Planococcus, for which the name Planococcus daihaiensis sp. nov. is proposed. The type strain is X10-3T (= CGMCC 1.60163T = KCTC 43688T).