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<p>Bacterial wilt caused by <i>Ralstonia pseudosolanacearum</i> is a major devastating soil-borne disease and seriously damages tomato crops. In this study, the strain <i>Bacillus velezensis</i> BB22 exhibited strong antagonistic activity against <i>R. pseudosolanacearum</i> MH01 in dual culture assays. In addition, <i>B. velezensis</i> BB22 produced a wide range of hydrolytic enzymes, promoted plant growth, and inhibited violacein pigment synthesis in <i>Chromobacterium violaceum</i> ATCC 12472<sup>T</sup>, indicating quorum quenching activity. Pot experiments further showed that tomato plants treated once or twice with <i>B. velezensis</i> BB22 and inoculated with <i>R. pseudosolanacearum</i> MH01 had disease severities of 53.13% and 45.31% at 5 days, respectively, compared with 89.85% in plants inoculated with <i>R. pseudosolanacearum</i> MH01 alone. These results demonstrate that <i>B. velezensis</i> BB22 inoculation can effectively reduce bacterial wilt severity and promote tomato growth. Maximum parsimony and maximum likelihood phylogenetic analyses based on 16S rRNA confirmed that strain BB22 (acc. number OP104226) belongs to <i>B. velezensis</i>. Genome sequencing confirmed and revealed that <i>B. velezensis</i> BB22 (acc. number GCA_051131645) consisted of a single circular chromosome of 3,919,343&#xa0;bp, containing 3,762 protein-coding genes, an average G + C content of 46.43%, and 13 biosynthetic gene clusters associated with secondary metabolite production, including bacillaene, bacillibactin, bacilysin, fengycin, mersacidin, macrolactin H, difficidin, surfactin, bacinapeptin, and butirosin A-B. The results indicate that <i>B. velezensis</i> BB22 is a promising biocontrol agent for managing bacterial wilt in tomato and for developing sustainable disease management strategies in the future.</p>

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Characteristics, Quorum Quenching Activity, and Whole Genome Sequence of Bacillus velezensis BB22, a Potential Biocontrol Agent against Bacterial Wilt of Tomato

  • 期刊论文

  • 发表日期：

    2026年2月19日

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  • Kanchana Srimai,
  • On-Uma Ruangwong,
  • Milan C. Samarakoon,
  • Angsana Akarapisan

摘要

Bacterial wilt caused by Ralstonia pseudosolanacearum is a major devastating soil-borne disease and seriously damages tomato crops. In this study, the strain Bacillus velezensis BB22 exhibited strong antagonistic activity against R. pseudosolanacearum MH01 in dual culture assays. In addition, B. velezensis BB22 produced a wide range of hydrolytic enzymes, promoted plant growth, and inhibited violacein pigment synthesis in Chromobacterium violaceum ATCC 12472T, indicating quorum quenching activity. Pot experiments further showed that tomato plants treated once or twice with B. velezensis BB22 and inoculated with R. pseudosolanacearum MH01 had disease severities of 53.13% and 45.31% at 5 days, respectively, compared with 89.85% in plants inoculated with R. pseudosolanacearum MH01 alone. These results demonstrate that B. velezensis BB22 inoculation can effectively reduce bacterial wilt severity and promote tomato growth. Maximum parsimony and maximum likelihood phylogenetic analyses based on 16S rRNA confirmed that strain BB22 (acc. number OP104226) belongs to B. velezensis. Genome sequencing confirmed and revealed that B. velezensis BB22 (acc. number GCA_051131645) consisted of a single circular chromosome of 3,919,343 bp, containing 3,762 protein-coding genes, an average G + C content of 46.43%, and 13 biosynthetic gene clusters associated with secondary metabolite production, including bacillaene, bacillibactin, bacilysin, fengycin, mersacidin, macrolactin H, difficidin, surfactin, bacinapeptin, and butirosin A-B. The results indicate that B. velezensis BB22 is a promising biocontrol agent for managing bacterial wilt in tomato and for developing sustainable disease management strategies in the future.