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<p>An actinomycete, designated strain H11422<sup>T</sup>, was isolated from sediment collected from Mahu Lake, Sichuan Province, P.R. China, and characterized by a polyphasic taxonomic approach. Strain H11422<sup>T</sup>, based on the 16&#xa0;S rRNA gene sequence analysis, exhibited the highest similarities to <i>Pseudonocardia acidicola</i> K10HN5<sup>T</sup> (98.5%), <i>Pseudonocardia bannensis</i> YIM63101<sup>T</sup> (97.9%), <i>Pseudonocardia nigra</i> ATK03<sup>T</sup> (97.8%) and <i>Pseudonocardia saturnea</i> IMSNU 20,052<sup>T</sup> (97.7%). Phylogenetic analysis based on the 16&#xa0;S rRNA gene sequence placed strain H11422<sup>T</sup> into the genus <i>Pseudonocardia</i>, where it formed a subclade with <i>Pseudonocardia acidicola</i> K10HN5<sup>T</sup>. Genomic comparison with the closest type strain revealed average nucleotide identity (ANI) value of 84.8%, average amino acid identity (AAI) value of 81.6%, and digital DNA-DNA hybridization (dDDH) value of 28.9%, all significantly below the accepted species delineation thresholds. The whole-cell hydrolysates of strain H11422<sup>T</sup> were composed of <i>meso</i>-diaminopimelic acid (<i>meso</i>-DAP) as the diagnostic diamino acid, and arabinose, galactose, glucose and ribose as the major sugars. Mycolic acids were not present. The only menaquinone was determined to be MK-8(H<sub>4</sub>). The phospholipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified phospholipids, two unidentified aminophospholipids and two unidentified lipids. The predominant fatty acid was <i>iso</i>-C<sub>16:0</sub>. Based on this comprehensive taxonomic evidence, strain H11422<sup>T</sup> represents a novel species within the genus <i>Pseudonocardia</i>, for which the name <i>Pseudonocardia mahuensis</i> sp. nov. is proposed. The type strain is H11422<sup>T</sup> (= JCM 34850<sup>T</sup> = CICC 25117<sup>T</sup>).</p>

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Pseudonocardia mahuensis sp. nov., Isolated from Sediment of Mahu Lake in China

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Chaolan Liu,
  • Liwei Zhou,
  • Zhuoxin Du,
  • Jianghua Li,
  • Lufeng Dan,
  • Yan Ge,
  • Guoqing Zhuang,
  • Yidong Guo

摘要

An actinomycete, designated strain H11422T, was isolated from sediment collected from Mahu Lake, Sichuan Province, P.R. China, and characterized by a polyphasic taxonomic approach. Strain H11422T, based on the 16 S rRNA gene sequence analysis, exhibited the highest similarities to Pseudonocardia acidicola K10HN5T (98.5%), Pseudonocardia bannensis YIM63101T (97.9%), Pseudonocardia nigra ATK03T (97.8%) and Pseudonocardia saturnea IMSNU 20,052T (97.7%). Phylogenetic analysis based on the 16 S rRNA gene sequence placed strain H11422T into the genus Pseudonocardia, where it formed a subclade with Pseudonocardia acidicola K10HN5T. Genomic comparison with the closest type strain revealed average nucleotide identity (ANI) value of 84.8%, average amino acid identity (AAI) value of 81.6%, and digital DNA-DNA hybridization (dDDH) value of 28.9%, all significantly below the accepted species delineation thresholds. The whole-cell hydrolysates of strain H11422T were composed of meso-diaminopimelic acid (meso-DAP) as the diagnostic diamino acid, and arabinose, galactose, glucose and ribose as the major sugars. Mycolic acids were not present. The only menaquinone was determined to be MK-8(H4). The phospholipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified phospholipids, two unidentified aminophospholipids and two unidentified lipids. The predominant fatty acid was iso-C16:0. Based on this comprehensive taxonomic evidence, strain H11422T represents a novel species within the genus Pseudonocardia, for which the name Pseudonocardia mahuensis sp. nov. is proposed. The type strain is H11422T (= JCM 34850T = CICC 25117T).