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<p>Two novel aerobic, rod-shaped, motile bacterial strains, designated as strains sgz302251<sup>T</sup> and sgz5001026<sup>T</sup>, were both isolated from paddy soils. 16S rRNA gene sequences analysis revealed that strain sgz302251<sup>T</sup> exhibited 98.3% similarity with its closest reference strain <i>Paenibacillus vietnamensis</i> N4<sup>T</sup>, while strain sgz5001026<sup>T</sup> had the highest similarity of 97.5% to <i>Ectobacillus polymachus</i> KACC 18242<sup>T</sup>. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains sgz302251<sup>T</sup>, sgz5001026<sup>T</sup> and their closest reference strains were 86.0% and 21.2%, 84.9% and 22.9%, respectively. The genomic DNA G + C contents of sgz302251<sup>T</sup> and sgz5001026<sup>T</sup> were 63.4% and 68.8%, respectively. Strain sgz302251<sup>T</sup> grew at 20–37&#xa0;°C (optimum 30&#xa0;°C), pH 5.0–9.0 (optimum 6.5–7.5) and tolerated 0–1.0% NaCl (optimum 0.5%, w/v), while strain sgz5001026<sup>T</sup> grew at 4–45&#xa0;°C (optimum 37&#xa0;°C), pH 5.0–9.0 (optimum 6.0) and 0–0.5% NaCl (optimum 0%). The quinone in both strains was MK-7 and major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The main fatty acids (&gt; 5%) of strain sgz302251<sup>T</sup> were anteiso-C<sub>15:0</sub>, anteiso-C<sub>17:0</sub>, and C<sub>16:0</sub>, whereas strain sgz5001026<sup>T</sup> included anteiso-C<sub>15:0</sub>, iso-C<sub>15:0</sub>, iso-C<sub>16:0</sub> and iso-C<sub>14:0</sub>. Based on the above evidence, the proposed names of the two novel species are <i>Paenibacillus praedii</i> sp. nov. (type strain sgz302251<sup>T</sup> = MCCC 1K09633<sup>T</sup> = KCTC 43808<sup>T</sup>), <i>Ectobacillus soli</i> sp. nov. (type strain sgz5001026<sup>T</sup> = MCCC 1K09373<sup>T</sup> = KCTC 25903<sup>T</sup>), respectively.</p>

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Two Novel Species of Paenibacillus praedii sp. nov. and Ectobacillus soil sp. nov.

  • 期刊论文

  • 发表日期：

    2025年12月29日

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  • Peng Gao,
  • Xie-Feng Lu,
  • Ling Yao,
  • Yuan-Jia Xu,
  • Shu-Yi Zhang,
  • Guo-Hong Liu,
  • Shun-Gui Zhou

摘要

Two novel aerobic, rod-shaped, motile bacterial strains, designated as strains sgz302251T and sgz5001026T, were both isolated from paddy soils. 16S rRNA gene sequences analysis revealed that strain sgz302251T exhibited 98.3% similarity with its closest reference strain Paenibacillus vietnamensis N4T, while strain sgz5001026T had the highest similarity of 97.5% to Ectobacillus polymachus KACC 18242T. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains sgz302251T, sgz5001026T and their closest reference strains were 86.0% and 21.2%, 84.9% and 22.9%, respectively. The genomic DNA G + C contents of sgz302251T and sgz5001026T were 63.4% and 68.8%, respectively. Strain sgz302251T grew at 20–37 °C (optimum 30 °C), pH 5.0–9.0 (optimum 6.5–7.5) and tolerated 0–1.0% NaCl (optimum 0.5%, w/v), while strain sgz5001026T grew at 4–45 °C (optimum 37 °C), pH 5.0–9.0 (optimum 6.0) and 0–0.5% NaCl (optimum 0%). The quinone in both strains was MK-7 and major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The main fatty acids (> 5%) of strain sgz302251T were anteiso-C15:0, anteiso-C17:0, and C16:0, whereas strain sgz5001026T included anteiso-C15:0, iso-C15:0, iso-C16:0 and iso-C14:0. Based on the above evidence, the proposed names of the two novel species are Paenibacillus praedii sp. nov. (type strain sgz302251T = MCCC 1K09633T = KCTC 43808T), Ectobacillus soli sp. nov. (type strain sgz5001026T = MCCC 1K09373T = KCTC 25903T), respectively.