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<p>The nitrogen-fixing bacterium <i>Kosakonia radicincitans</i> GXGL-4A can produce siderophore to sequester iron, which is essential for survival and bacteriostasis. In this investigation, a mutant, M246-2, resulting from Tn5 insertion mutagenesis of the strain GXGL-4A, was found to have lost the ability to synthesize siderophores. Whole-genome sequencing of the mutant M246-2 revealed that the insertion site is within the nucleotide sequence of the <i>gua</i>A gene encoding glutamine-hydrolyzing guanosine monophosphate (GMP) synthase. The deletion of the <i>gua</i>A gene (<i>∆gua</i>A) resulted in the loss of the ability to produce siderophores in the strain GXGL-4A and a significant slowdown in the bacterial growth curve. Moreover, the intracellular GMP contents in the strains M246-2 and <i>∆gua</i>A were statistically significantly reduced compared to the wild-type strain GXGL-4A. The bacterial functional complementation and <i>gua</i>A gene deletion experiments revealed that the <i>gua</i>A gene modulated the siderophore synthesis in the strain GXGL-4A. The results of differential transcriptome analysis showed that the transcription levels of genes related to the siderophore synthesis in the mutant M246-2 had no significant changes compared to the wild-type strain GXGL-4A, suggesting that the <i>gua</i>A gene may modulate the siderophore synthesis of the strain GXGL-4A through a complex mechanism at the non-transcriptional level.</p>

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Glutamine-hydrolyzing GMP Synthase Gene (guaA) Regulates Siderophore Synthesis in the Nitrogen-fixing Bacterium (NFB) Kosakonia radicincitans GXGL-4A

  • 期刊论文

  • 发表日期：

    2026年1月12日

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  • Er-Xing Wang,
  • Bao-Yun Feng,
  • Ya-Ting Zhang,
  • Meng-Ting Zhang,
  • Lu-Rong Xu,
  • Yan-Wen Xue,
  • Yun-Peng Chen

摘要

The nitrogen-fixing bacterium Kosakonia radicincitans GXGL-4A can produce siderophore to sequester iron, which is essential for survival and bacteriostasis. In this investigation, a mutant, M246-2, resulting from Tn5 insertion mutagenesis of the strain GXGL-4A, was found to have lost the ability to synthesize siderophores. Whole-genome sequencing of the mutant M246-2 revealed that the insertion site is within the nucleotide sequence of the guaA gene encoding glutamine-hydrolyzing guanosine monophosphate (GMP) synthase. The deletion of the guaA gene (∆guaA) resulted in the loss of the ability to produce siderophores in the strain GXGL-4A and a significant slowdown in the bacterial growth curve. Moreover, the intracellular GMP contents in the strains M246-2 and ∆guaA were statistically significantly reduced compared to the wild-type strain GXGL-4A. The bacterial functional complementation and guaA gene deletion experiments revealed that the guaA gene modulated the siderophore synthesis in the strain GXGL-4A. The results of differential transcriptome analysis showed that the transcription levels of genes related to the siderophore synthesis in the mutant M246-2 had no significant changes compared to the wild-type strain GXGL-4A, suggesting that the guaA gene may modulate the siderophore synthesis of the strain GXGL-4A through a complex mechanism at the non-transcriptional level.