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<p>A Gram-stain-positive, rod-shaped, endospore-forming, immotile and aerobic bacterial strain, designated as SYSU BS000544<sup>T</sup>, was isolated from a black soil sample in Harbin, Heilongjiang province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU BS000544<sup>T</sup> belongs to the genus <i>Brevibacillus</i>, and showed the highest sequence similarity to <i>Brevibacillus ginsengisoli</i> KCTC 13938<sup>T</sup> (98.09%). Strain SYSU BS000544<sup>T</sup> was found to grow at 25–37&#xa0;°C (optimum, 30&#xa0;°C) and pH 6.0–7.0 (optimum, pH 6.5) and in the presence of 0% (w/v) NaCl. Polar lipids comprised of phosphophatidylinositol, phosphatidylethanolamine, one unidentified aminophospholipid, and two unidentified polar lipids. The major cellular fatty acids (&gt; 5%) were iso-C<sub>17:1</sub> <i>ω</i>9<i>c</i> and/or 10-methyl C<sub>16:0</sub>, iso-C<sub>16:0</sub>, iso-C<sub>17:0</sub>, iso-C<sub>14:0</sub> and anteiso-C<sub>15:0</sub>. The predominant respiratory quinone was MK-7. The genomic G + C content was 48.84% based on the whole genome analysis. The average nucleotide identity (≤ 78.88%) and digital DNA-DNA hybridization (≤ 15.7%) values between strain SYSU BS000544<sup>T</sup> and other members of the genus <i>Brevibacillus</i> were all lower than the threshold values recommended for distinguishing novel species. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain SYSU BS000544<sup>T</sup> represents a novel species of the genus <i>Brevibacillus</i>, for which the name <i>Brevibacillus nigriterrae</i> sp. nov. is proposed. The type strain is SYSU BS000544<sup>T</sup> (= MCCC 1K09332<sup>T</sup> = KCTC 43752<sup>T</sup>).</p>

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Brevibacillus nigriterrae sp. nov., a Novel Denitrification Bacterium Isolated from Black Soil

  • 期刊论文

  • 发表日期：

    2025年12月12日

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  • Jun Xiao,
  • Xin-Ting Yuan,
  • Le-Bin Chen,
  • Yu-Ting OuYang,
  • Xin Huang,
  • Jia-Hao Tan,
  • Jun Liu,
  • Shuang Wang,
  • Jian-Yu Jiao,
  • Wen-Jun Li

摘要

A Gram-stain-positive, rod-shaped, endospore-forming, immotile and aerobic bacterial strain, designated as SYSU BS000544T, was isolated from a black soil sample in Harbin, Heilongjiang province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU BS000544T belongs to the genus Brevibacillus, and showed the highest sequence similarity to Brevibacillus ginsengisoli KCTC 13938T (98.09%). Strain SYSU BS000544T was found to grow at 25–37 °C (optimum, 30 °C) and pH 6.0–7.0 (optimum, pH 6.5) and in the presence of 0% (w/v) NaCl. Polar lipids comprised of phosphophatidylinositol, phosphatidylethanolamine, one unidentified aminophospholipid, and two unidentified polar lipids. The major cellular fatty acids (> 5%) were iso-C17:1 ω9c and/or 10-methyl C16:0, iso-C16:0, iso-C17:0, iso-C14:0 and anteiso-C15:0. The predominant respiratory quinone was MK-7. The genomic G + C content was 48.84% based on the whole genome analysis. The average nucleotide identity (≤ 78.88%) and digital DNA-DNA hybridization (≤ 15.7%) values between strain SYSU BS000544T and other members of the genus Brevibacillus were all lower than the threshold values recommended for distinguishing novel species. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain SYSU BS000544T represents a novel species of the genus Brevibacillus, for which the name Brevibacillus nigriterrae sp. nov. is proposed. The type strain is SYSU BS000544T (= MCCC 1K09332T = KCTC 43752T).