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<p>A Gram-stain negative, aerobic, non-motile, coccoid bacterium, designated IMCC34717<sup>T</sup>, was isolated from a lotus wetland in the Republic of Korea. Strain IMCC34717<sup>T</sup> grew at 15–38&#xa0;°C (optimum, 30&#xa0;°C), at pH 6.0–9.0 (optimum, pH 7.0), and in the presence of 0–0.7% (w/v) NaCl (optimum, 0.5% NaCl). Phylogenetic analysis of 16S rRNA genes placed strain IMCC34717<sup>T</sup> within the family <i>Opitutaceae</i>, showing highest sequence similarity (95.2%) to <i>Nibricoccus aquaticus</i> HZ-65<sup>T</sup>. The complete whole-genome was 3.56 Mbp in size with a DNA G + C content of 61.8%. Overall genomic relatedness indices between strain IMCC34717<sup>T</sup> and members of the family <i>Opitutaceae</i> were at or below the proposed genus thresholds, and the genome-based phylogenetic trees revealed a distinct phylogenetic separation from known genera, supporting its recognition as a representative of a novel genus. Genomic analysis revealed pathways for nitrogen, sulfur, and carbon metabolism, including unique amylase-related genes absent from closely related species, <i>Nibricoccus aquaticus</i>, suggesting ecological specialization of the strain. The major respiratory quinone was menaquinone-7 (MK-7), and the predominant cellular fatty acids were iso-C<sub>14:0</sub> (28.1%), C<sub>16:0</sub> (25.1%), and anteiso-C<sub>15:0</sub> (21.6%). The major polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, and three unidentified lipids. On the basis of phylogenetic, genomic, and phenotypic evidence, strain IMCC34717<sup>T</sup> is considered to represent a novel genus and species of the family <i>Opitutaceae</i>, for which the name <i>Lotipaludicoccus stagni</i> gen. nov., sp. nov. is proposed. The type strain is IMCC34717<sup>T</sup> (= KCTC 82067<sup>T</sup> = KACC 23134<sup>T</sup> = NBRC 113911<sup>T</sup>).</p>

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Lotipaludicoccus stagni gen. nov., sp. nov., within the Family Opitutaceae, Isolated from a Lotus Wetland

  • 期刊论文

  • 发表日期：

    2025年12月12日

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  • Miri S. Park,
  • Hyerim Cho,
  • Yeonjung Lim,
  • Ilnam Kang,
  • Jang-Cheon Cho

摘要

A Gram-stain negative, aerobic, non-motile, coccoid bacterium, designated IMCC34717T, was isolated from a lotus wetland in the Republic of Korea. Strain IMCC34717T grew at 15–38 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0), and in the presence of 0–0.7% (w/v) NaCl (optimum, 0.5% NaCl). Phylogenetic analysis of 16S rRNA genes placed strain IMCC34717T within the family Opitutaceae, showing highest sequence similarity (95.2%) to Nibricoccus aquaticus HZ-65T. The complete whole-genome was 3.56 Mbp in size with a DNA G + C content of 61.8%. Overall genomic relatedness indices between strain IMCC34717T and members of the family Opitutaceae were at or below the proposed genus thresholds, and the genome-based phylogenetic trees revealed a distinct phylogenetic separation from known genera, supporting its recognition as a representative of a novel genus. Genomic analysis revealed pathways for nitrogen, sulfur, and carbon metabolism, including unique amylase-related genes absent from closely related species, Nibricoccus aquaticus, suggesting ecological specialization of the strain. The major respiratory quinone was menaquinone-7 (MK-7), and the predominant cellular fatty acids were iso-C14:0 (28.1%), C16:0 (25.1%), and anteiso-C15:0 (21.6%). The major polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, and three unidentified lipids. On the basis of phylogenetic, genomic, and phenotypic evidence, strain IMCC34717T is considered to represent a novel genus and species of the family Opitutaceae, for which the name Lotipaludicoccus stagni gen. nov., sp. nov. is proposed. The type strain is IMCC34717T (= KCTC 82067T = KACC 23134T = NBRC 113911T).