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<p>Two Gram-reaction-positive bacterial strains, designated BWT-B31<sup>T</sup> and BWR-S6Y, were isolated from larvae of <i>Allomyrina dichotoma,</i> and their taxonomic positions were examined by a polyphasic approach. Both strains were found to be Gram-reaction-positive, strictly aerobic, short rods and grew at 10–37&#xa0;°C, pH 5.0–10.0 and in the presence of up to 5% (w/v) NaCl concentration (optimum, 0–1%). Strain BWT-B31<sup>T</sup> showed the highest 16S rRNA gene sequence similarity (98.1%) with <i>Microbacterium mangrovi</i>, followed by 97.9% with <i>Microbacterium immunditiarum</i>, while strain BWR-S6Y shared 100% sequence identity with <i>Microbacterium hydrothermale</i>, followed by 99.9% sequence similarity with <i>Microbacterium galbum</i>. In the core genome-based phylogenetic tree, strain BWT-B31<sup>T</sup> formed a clade with <i>Microbacterium immunditiarum</i> and <i>Microbacterium mangrovi</i>, with orthologous average nucleotide identities of 81.1% and 76.2%, respectively. Strain BWR-S6Y showed the same topology with that of 16S rRNA gene phylogeny, with an orthologous average nucleotide identity of 96.8% and a digital DNA–DNA hybridization value of 72.0% with <i>Microbacterium hydrothermale</i>. A combination of the phenotypic distinctness and overall genomic relatedness indices supports that strain BWR-S6Y is a strain of <i>Microbacterium hydrothermale</i>, while strain BWT-B31<sup>T</sup> represents a new species of the genus <i>Microbacterium</i>, for which the name <i>Microbacterium insectihabitans</i> sp. nov. (type strain, BWT-B31<sup>T</sup> = KACC 22261<sup>T</sup> = DSM 112528<sup>T</sup> = NBRC 115128<sup>T</sup>) is proposed.</p>

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Microbacterium insectihabitans sp. nov., Isolated from Larvae of Allomyrina dichotoma

  • 期刊论文

  • 发表日期：

    2025年10月24日

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  • Soon Dong Lee,
  • Hong Lim Yang,
  • In Seop Kim

摘要

Two Gram-reaction-positive bacterial strains, designated BWT-B31T and BWR-S6Y, were isolated from larvae of Allomyrina dichotoma, and their taxonomic positions were examined by a polyphasic approach. Both strains were found to be Gram-reaction-positive, strictly aerobic, short rods and grew at 10–37 °C, pH 5.0–10.0 and in the presence of up to 5% (w/v) NaCl concentration (optimum, 0–1%). Strain BWT-B31T showed the highest 16S rRNA gene sequence similarity (98.1%) with Microbacterium mangrovi, followed by 97.9% with Microbacterium immunditiarum, while strain BWR-S6Y shared 100% sequence identity with Microbacterium hydrothermale, followed by 99.9% sequence similarity with Microbacterium galbum. In the core genome-based phylogenetic tree, strain BWT-B31T formed a clade with Microbacterium immunditiarum and Microbacterium mangrovi, with orthologous average nucleotide identities of 81.1% and 76.2%, respectively. Strain BWR-S6Y showed the same topology with that of 16S rRNA gene phylogeny, with an orthologous average nucleotide identity of 96.8% and a digital DNA–DNA hybridization value of 72.0% with Microbacterium hydrothermale. A combination of the phenotypic distinctness and overall genomic relatedness indices supports that strain BWR-S6Y is a strain of Microbacterium hydrothermale, while strain BWT-B31T represents a new species of the genus Microbacterium, for which the name Microbacterium insectihabitans sp. nov. (type strain, BWT-B31T = KACC 22261T = DSM 112528T = NBRC 115128T) is proposed.