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<p>Two aerobic, non-motile, Gram-stain-negative strains, designated SN3-11ᵀ and SN6-1ᵀ, were isolated from marine algae. Both strains were oxidase- and catalase-positive, with rod-shaped morphology. Strain SN3-11ᵀ grew optimally at 25&#xa0;℃, pH 7.0–8.0, and 2.0–5.0% (w/v) NaCl, while strain SN6-1ᵀ showed optimal growth at 25&#xa0;℃, pH 8.0, and 2.0–3.0% NaCl. Both strains contained ubiquinone-10 as the sole respiratory quinone, and their major fatty acids were summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>6<i>c</i>), C<sub>16:0</sub>, and C<sub>19:0</sub> cyclo <i>ω</i>8<i>c</i>. Phosphatidylcholine and phosphatidylglycerol were dominant polar lipids, with diphosphatidylglycerol additionally present in SN3-11ᵀ. Strains SN3-11ᵀ and SN6-1ᵀ had genome sizes of 4,762 Kb and 4,157 Kb with G + C contents of 61.3% and 62.0%, respectively. They shared 96.9% 16S rRNA gene sequence similarity, 77.0% average nucleotide identity (ANI), and 20.2% digital DNA-DNA hybridization (dDDH), supporting their classification as distinct species. Their closest relative, <i>Tateyamaria armeniaca</i> KMU-156ᵀ, exhibited 16S rRNA gene sequence similarities of 98.6% to SN3-11ᵀ and 97.7% to SN6-1ᵀ. Phylogenetic trees based on 16S rRNA and whole-genome sequences placed both strains in distinct lineages within <i>Tateyamaria</i>. ANI and dDDH values between the two isolates and other <i>Tateyamaria</i> species were &lt; 78.5% and &lt; 21.0%, respectively. Strains SN3-11ᵀ and SN6-1ᵀ harbored genes encoding diverse carbohydrate-active enzymes and biosynthetic pathways for lycopene, pantothenate, and riboflavin, suggesting potential symbiotic roles with algal hosts. Based on phenotypic, chemotaxonomic, and genomic characteristics, SN3-11ᵀ and SN6-1ᵀ represent two novel species, <i>Tateyamaria algicola</i> sp. nov. (SN3-11ᵀ = KACC 23689ᵀ = JCM 36649ᵀ) and <i>Tateyamaria rhodophyticola</i> sp. nov. (SN6-1ᵀ = KACC 23127ᵀ = JCM 35962ᵀ).</p>

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Description of Tateyamaria algicola sp. nov. and Tateyamaria rhodophyticola sp. nov., Isolated from Marine Algae

  • 期刊论文

  • 发表日期：

    2025年10月9日

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  • Byeong Jun Choi,
  • Jeong Min Kim,
  • Hülya Bayburt,
  • Dae Gyu Choi,
  • Seo Hui Choi,
  • Che Ok Jeon

摘要

Two aerobic, non-motile, Gram-stain-negative strains, designated SN3-11ᵀ and SN6-1ᵀ, were isolated from marine algae. Both strains were oxidase- and catalase-positive, with rod-shaped morphology. Strain SN3-11ᵀ grew optimally at 25 ℃, pH 7.0–8.0, and 2.0–5.0% (w/v) NaCl, while strain SN6-1ᵀ showed optimal growth at 25 ℃, pH 8.0, and 2.0–3.0% NaCl. Both strains contained ubiquinone-10 as the sole respiratory quinone, and their major fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, and C19:0 cyclo ω8c. Phosphatidylcholine and phosphatidylglycerol were dominant polar lipids, with diphosphatidylglycerol additionally present in SN3-11ᵀ. Strains SN3-11ᵀ and SN6-1ᵀ had genome sizes of 4,762 Kb and 4,157 Kb with G + C contents of 61.3% and 62.0%, respectively. They shared 96.9% 16S rRNA gene sequence similarity, 77.0% average nucleotide identity (ANI), and 20.2% digital DNA-DNA hybridization (dDDH), supporting their classification as distinct species. Their closest relative, Tateyamaria armeniaca KMU-156ᵀ, exhibited 16S rRNA gene sequence similarities of 98.6% to SN3-11ᵀ and 97.7% to SN6-1ᵀ. Phylogenetic trees based on 16S rRNA and whole-genome sequences placed both strains in distinct lineages within Tateyamaria. ANI and dDDH values between the two isolates and other Tateyamaria species were < 78.5% and < 21.0%, respectively. Strains SN3-11ᵀ and SN6-1ᵀ harbored genes encoding diverse carbohydrate-active enzymes and biosynthetic pathways for lycopene, pantothenate, and riboflavin, suggesting potential symbiotic roles with algal hosts. Based on phenotypic, chemotaxonomic, and genomic characteristics, SN3-11ᵀ and SN6-1ᵀ represent two novel species, Tateyamaria algicola sp. nov. (SN3-11ᵀ = KACC 23689ᵀ = JCM 36649ᵀ) and Tateyamaria rhodophyticola sp. nov. (SN6-1ᵀ = KACC 23127ᵀ = JCM 35962ᵀ).