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<p>Three Gram-stain-negative, strictly aerobic, cream-colored, catalase- and oxidase-positive bacterial strains, designated 2205SS24-7<sup>T</sup>, 2304DJ69-11<sup>T</sup>, and 2012CJ15-1<sup>T</sup>, were isolated from marine sponges collected in the Republic of Korea. The three novel strains grew optimally at 25–28&#xa0;°C, pH 7.0, and 2–3% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences revealed that the three novel strains clustered together with <i>Ruegeria spongiae</i> 2012CJ41-6<sup>T</sup>, forming a distinct clade within the genus <i>Ruegeria</i>. The 16S rRNA gene sequence similarities with <i>R. spongiae</i> were 97.6, 98.6, and 98.9%, respectively. Draft genome sequencing indicated genome sizes ranging from 5.2 to 6.1&#xa0;Mb with DNA G+C contents between 59.0 and 60.5%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the three novel strains ranged from 85.3–90.9% and 29.6–43.1%, respectively, indicating that each strain represents a separate species. Furthermore, ANI and dDDH values between the three novel strains and the type strains <i>R</i>. <i>spongiae</i> 2012CJ41-6<sup>T</sup>, <i>R</i>. <i>mediterranea</i> M17<sup>T</sup>, and <i>R</i>. <i>litorea</i> R-37<sup>T</sup> were ≤ 90.9% and ≤ 43.1%, respectively, both below the proposed thresholds for species delineation. The three novel strains possessed ubiquinone-10 as the major isoprenoid quinone and summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>6<i>c</i>) as the major fatty acid. The major polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Taken together, these results support the proposal that strain 2205SS24-7<sup>T</sup> (= KACC 23237<sup>T</sup> = LMG 33064<sup>T</sup>), 2304DJ69-11<sup>T</sup> (= KACC 23805<sup>T</sup> = MCCC 1K09534<sup>T</sup>), and 2012CJ15-1<sup>T</sup> (= KACC 23842<sup>T</sup> = MCCC 1K09526<sup>T</sup>) represent three novel species within the genus <i>Ruegeria</i>, for which the names <i>Ruegeria discodermiae</i> sp. nov., <i>Ruegeria jejuensis</i> sp. nov., and <i>Ruegeria hyattellae</i> sp. nov. are proposed, respectively.</p>

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Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov., Isolated from Marine Sponges

  • 期刊论文

  • 发表日期：

    2025年9月16日

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  • Yea-Lin Moon,
  • Kyung Hyun Kim,
  • Ji-Yoon Shin,
  • Jin-Sook Park

摘要

Three Gram-stain-negative, strictly aerobic, cream-colored, catalase- and oxidase-positive bacterial strains, designated 2205SS24-7T, 2304DJ69-11T, and 2012CJ15-1T, were isolated from marine sponges collected in the Republic of Korea. The three novel strains grew optimally at 25–28 °C, pH 7.0, and 2–3% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences revealed that the three novel strains clustered together with Ruegeria spongiae 2012CJ41-6T, forming a distinct clade within the genus Ruegeria. The 16S rRNA gene sequence similarities with R. spongiae were 97.6, 98.6, and 98.9%, respectively. Draft genome sequencing indicated genome sizes ranging from 5.2 to 6.1 Mb with DNA G+C contents between 59.0 and 60.5%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the three novel strains ranged from 85.3–90.9% and 29.6–43.1%, respectively, indicating that each strain represents a separate species. Furthermore, ANI and dDDH values between the three novel strains and the type strains R. spongiae 2012CJ41-6T, R. mediterranea M17T, and R. litorea R-37T were ≤ 90.9% and ≤ 43.1%, respectively, both below the proposed thresholds for species delineation. The three novel strains possessed ubiquinone-10 as the major isoprenoid quinone and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) as the major fatty acid. The major polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Taken together, these results support the proposal that strain 2205SS24-7T (= KACC 23237T = LMG 33064T), 2304DJ69-11T (= KACC 23805T = MCCC 1K09534T), and 2012CJ15-1T (= KACC 23842T = MCCC 1K09526T) represent three novel species within the genus Ruegeria, for which the names Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov. are proposed, respectively.