<p>A novel lactic acid bacterium, OKN36<sup>T</sup>, was isolated from the bark of <i>Entada phaseoloides</i> on Amami Island in Japan. Strain OKN36<sup>T</sup> is characterized as a Gram-stain-positive, non-motile, catalase-negative, heterofermentative, and rod-shaped bacterium. The strain grew at temperatures ranging from 10 to 37&#xa0;°C, in a pH range of 4.0–8.0, and in the presence of 0–6.5% (w/v) NaCl. Phylogenetic analysis using the 16S rRNA gene analysis revealed that strain OKN36<sup>T</sup> belongs to the <i>Furfurilactobacillus</i> group. The average nucleotide identity (ANI) and the digital DNA–DNA hybridization (dDDH) values between strain OKN36<sup>T</sup> and type strains of validly published <i>Furfurilactobacillus</i> species were 71.83–73.85% and 19.7–20.6%, respectively. These values are below the cutoff of species differentiation, which is 95–96% for ANI and 70% for dDDH. Based on these data, we propose a novel species, named <i>Furfurilactobacillus entadae</i> sp. nov., with the type strain OKN36<sup>T</sup> (= JCM 37107<sup>T</sup> = DSM 118293<sup>T</sup>).</p>

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Furfurilactobacillus entadae sp. nov., Isolated from Bark of Entada phaseoloides

  • Shunya Suzuki,
  • Karin Okano,
  • Mizuna Tamaki,
  • Yoshimasa Tsujii,
  • Akihito Endo,
  • Akinobu Kajikawa

摘要

A novel lactic acid bacterium, OKN36T, was isolated from the bark of Entada phaseoloides on Amami Island in Japan. Strain OKN36T is characterized as a Gram-stain-positive, non-motile, catalase-negative, heterofermentative, and rod-shaped bacterium. The strain grew at temperatures ranging from 10 to 37 °C, in a pH range of 4.0–8.0, and in the presence of 0–6.5% (w/v) NaCl. Phylogenetic analysis using the 16S rRNA gene analysis revealed that strain OKN36T belongs to the Furfurilactobacillus group. The average nucleotide identity (ANI) and the digital DNA–DNA hybridization (dDDH) values between strain OKN36T and type strains of validly published Furfurilactobacillus species were 71.83–73.85% and 19.7–20.6%, respectively. These values are below the cutoff of species differentiation, which is 95–96% for ANI and 70% for dDDH. Based on these data, we propose a novel species, named Furfurilactobacillus entadae sp. nov., with the type strain OKN36T (= JCM 37107T = DSM 118293T).