Scholarlink

<p>A Gram-stain-positive actinomycete, designated strain 1LY6.1<sup>T</sup>, was isolated from the phylloplane of a mangrove plant (<i>Rhizophora apiculata</i> Blume). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was most closely related to <i>Pseudonocardia nantongensis</i> KLBMP 1282<sup>T</sup> (98.5%). The strain formed well-developed, branched substrate and aerial mycelium that fragmented into oval to rod-shaped and having smooth surfaces spores. The chemotaxonomic and morphological characteristics of strain 1LY6.1<sup>T</sup> were consistent with members of the genus <i>Pseudonocardia</i>, including <i>meso</i>-diaminopimelic acid as the diagnostic diamino acid. Major cellular fatty acids (&gt; 10% of total fatty acids) were&#xa0;<i>iso-</i>C<sub>16:0</sub> and summed feature 9 (10-methyl C<sub>16:0</sub> and/or <i>iso</i>-C<sub>17:1</sub> ω9c), and MK-8(H<sub>4</sub>) as the predominant menaquinone. However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the draft genome of this strain and its closely related type strain were 80.94 and 24.5%, respectively, which were below the proposed threshold values (95–96% for ANI and 70% for dDDH) used for species discrimination. Based on chemotaxonomic, phylogenetic, phenotypic, and genomic evidence obtained, we describe a novel <i>Pseudonocardia</i> species, for which the name <i>Pseudonocardia phyllosphaerae</i> sp. nov. is proposed. The type strain is 1LY6.1<sup>T</sup> (= TBRC 19001<sup>T</sup> = NBRC 116933<sup>T</sup>).</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Pseudonocardia phyllosphaerae sp. nov., a Novel Actinomycete Isolated from Phylloplane of Rhizophora apiculata Blume

  • 期刊论文

  • 发表日期：

    2025年8月18日

查看全文
  • Chaiyasit Khanoonkon,
  • Waranya Butdee,
  • Chanwit Suriyachadkun,
  • Nantana Srisuk,
  • Kannika Duangmal

摘要

A Gram-stain-positive actinomycete, designated strain 1LY6.1T, was isolated from the phylloplane of a mangrove plant (Rhizophora apiculata Blume). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was most closely related to Pseudonocardia nantongensis KLBMP 1282T (98.5%). The strain formed well-developed, branched substrate and aerial mycelium that fragmented into oval to rod-shaped and having smooth surfaces spores. The chemotaxonomic and morphological characteristics of strain 1LY6.1T were consistent with members of the genus Pseudonocardia, including meso-diaminopimelic acid as the diagnostic diamino acid. Major cellular fatty acids (> 10% of total fatty acids) were iso-C16:0 and summed feature 9 (10-methyl C16:0 and/or iso-C17:1 ω9c), and MK-8(H4) as the predominant menaquinone. However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the draft genome of this strain and its closely related type strain were 80.94 and 24.5%, respectively, which were below the proposed threshold values (95–96% for ANI and 70% for dDDH) used for species discrimination. Based on chemotaxonomic, phylogenetic, phenotypic, and genomic evidence obtained, we describe a novel Pseudonocardia species, for which the name Pseudonocardia phyllosphaerae sp. nov. is proposed. The type strain is 1LY6.1T (= TBRC 19001T = NBRC 116933T).