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<p>A Gram-stain-negative, rod-shaped bacterial strain, designated GZD-96<sup>T</sup>, characterized by strictly aerobic metabolism, was isolated from tidal flat collected at Sanbei Shoal, Ningbo, China. This strictly aerobic strain formed orange, round, convex colonies on marine agar at 37&#xa0;°C. It exhibited growth at 10–40&#xa0;°C (optimum: 37&#xa0;°C), 0–7% NaCl (optimum: 1%), and pH 5.5–9.5 (optimum: pH 8.0). Microscopic examination revealed short rod-shaped cells measuring 0.4–0.5&#xa0;μm in width and 1.0–1.1&#xa0;μm in length. The respiratory quinone profile of strain GZD-96<sup>T</sup> was dominated by menaquinone-6. Polar lipids comprised phosphatidylethanolamine, additional lipids, identified amino phospholipids, glycolipids, and amino lipids. The predominant fatty acids (&gt; 10%) included Summed feature 3 (comprising C<sub>16:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>16:1</sub> <i>ω</i>6<i>c</i>), iso-C<sub>15:0</sub>, C<sub>16:0</sub>, and iso-C<sub>17:0</sub> 3-OH. The genomic DNA of strain GZD-96<sup>T</sup> exhibited a G + C content of 39.4%, as determined by genome sequence analysis. Phylogenetic analysis based on the 16S rRNA gene sequence indicated the closest relative to be <i>Arenibacter troitsensis</i> KMM 3674<sup>T</sup>, with a sequence similarity of 94.7%. The average nucleotide identity (ANI), <i>digit</i> DNA–DNA hybridization (<i>d</i>DDH), and average amino acid identity (AAI) values between strain GZD-96<sup>T</sup> and closely related taxa were 69.79–70.54%, 16.80–18.20%, and 65.57–67.83%, respectively. Based on phylogenetic, chemotaxonomic, and genomic evidence, strain GZD-96<sup>T</sup> is proposed to represent a novel genus and species within the family <i>Flavobacteriaceae</i>, for which the name <i>Aurantibrevibacter litoralis</i> gen. nov., sp. nov. is proposed. The type strain is GZD-96<sup>T</sup> (= MCCC 1K08935<sup>T</sup> = KCTC 102227<sup>T</sup>).</p>

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Aurantibrevibacter litoralis gen. nov. sp. nov. a Novel Member in the Family Flavobacteriaceae Isolated from Sediment of Tidal Flat Located in Ningbo, China

  • 期刊论文

  • 发表日期：

    2025年7月30日

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  • Yun-Fei Cao,
  • Dong-Yan He,
  • Yu-Ruo Wang,
  • Ke Cao,
  • Yang Li,
  • Jia-Wei Gao,
  • Xing-Cheng Wang,
  • Lin Xu,
  • Cong Sun,
  • Xiao-Yun Yu

摘要

A Gram-stain-negative, rod-shaped bacterial strain, designated GZD-96T, characterized by strictly aerobic metabolism, was isolated from tidal flat collected at Sanbei Shoal, Ningbo, China. This strictly aerobic strain formed orange, round, convex colonies on marine agar at 37 °C. It exhibited growth at 10–40 °C (optimum: 37 °C), 0–7% NaCl (optimum: 1%), and pH 5.5–9.5 (optimum: pH 8.0). Microscopic examination revealed short rod-shaped cells measuring 0.4–0.5 μm in width and 1.0–1.1 μm in length. The respiratory quinone profile of strain GZD-96T was dominated by menaquinone-6. Polar lipids comprised phosphatidylethanolamine, additional lipids, identified amino phospholipids, glycolipids, and amino lipids. The predominant fatty acids (> 10%) included Summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), iso-C15:0, C16:0, and iso-C17:0 3-OH. The genomic DNA of strain GZD-96T exhibited a G + C content of 39.4%, as determined by genome sequence analysis. Phylogenetic analysis based on the 16S rRNA gene sequence indicated the closest relative to be Arenibacter troitsensis KMM 3674T, with a sequence similarity of 94.7%. The average nucleotide identity (ANI), digit DNA–DNA hybridization (dDDH), and average amino acid identity (AAI) values between strain GZD-96T and closely related taxa were 69.79–70.54%, 16.80–18.20%, and 65.57–67.83%, respectively. Based on phylogenetic, chemotaxonomic, and genomic evidence, strain GZD-96T is proposed to represent a novel genus and species within the family Flavobacteriaceae, for which the name Aurantibrevibacter litoralis gen. nov., sp. nov. is proposed. The type strain is GZD-96T (= MCCC 1K08935T = KCTC 102227T).